Sequences from confirmation amplicon


Confirmation sequences for line 291A10 and hit At4g18030 are shown.
Line ID 291A10
Locus At4g18030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10015219
Primers used for PCR LB14 - 8474, show primer details
   
Sequence Name 44-K033737-022-291-A10C-8409
GenBank Accession LN505092 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K033737-022-291-A10C-8409
TTTACAATTGAATATATCCTGATCCGGACATTGCTGATCTATCTCTACTCGTTGTGGTTG
GTTTATGTTGTTTCTTCTATCTTCTTGGAGCATGGCAAAAGAGTGGGTTTGGTAAAGGAG
ATAGCATAGCTATGGAGATTACAAAGCAAGCGCAGTGTACTGACATTGTCACTGATCTTG
ATTTTGAACCTCATCACAACACAGTGAAGATCCCACATAAAGCTGATCCCAAACCTGTTT
CTTTCAAACCGTGTGATGTGAAGCTCAAGGATTACACGCCTTGTCAAGAGCAAGACCGAG
CTATGAAGTTCCCGAGAGAGAACATGATTTACAGAGAGAGACATTGTCCTCCTGATAATG
AGAAGCTGCGTTGTCTTGTTCCAGCTCCTAAAGGGTATATGACTCCTTTCCCTTGGCCTA
AAAGCAGAGATTATGTTCACTATGCTAATGCTCCTTTCAAGAGCTTGACTGTCGAAAAAG
CTGGACAGAATTGGGTTCAGTTTCAAGGGAATGTGTTTAAATTCCCTGGTGGAGGAACTA
TGTTTCCTCAAGGTGCTGATGCGTATATTGAAGAGCTAGCTTCTGTTATCCCTATCAAAG
ATGGGTCCTGTTA
   
Sequence Name 46-K033737-022-291-A10C-LB14
GenBank Accession LN505783 [GenBank]
Sequencing primer gene specific primer, LB14: 5'-CTAACAGAGCCATCTTTGATAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K033737-022-291-A10C-LB14
ACGCATCAGCACCTTGAGGAAACATAGTTCCTCCACCAGGGAATTTAAACACATTCCCTT
GAAACTGAACCCAATTCTGTCCAGCTTTTTCGACAGTCAAGCTCTTGAAAGGAGCATTAG
CATAGTGAACATAATCTCTGCTTTTAGGCCAAGGGAAAGGAGTCATATACCCTTTAGGAG
CTGGAACAAGACAACGCAGCTTCTCATTATCAGGAGGACAATGTCTCTCTCTGTAAATCA
TGTTCTCTCTCGGGAACTTCATAGCTCGGTCTTGCTCTTGACAAGGCGTGTAATCCTTGA
GCTTCACATCACACGGTTTGAAAGAAACAGGTTTGGGATCAGCTTTATGTGGGATCTTCA
CTGTGTTGTGATGAGGTTCAAAATCAAGATCAGTGACAATGTCAGTACACTGCGCTTGCT
TTGTAATCTCCATAGCTATGCTATCTCCTTTACCAAACCCACTCTTTTGCCATGCTCCAA
GAAGATAGAAGAAACAACATAAACCAACCACAACGAGTAGAGATAGATCAGCAATGTCCG
GATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAGATCT
TTATNTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11