Sequences from confirmation amplicon


Confirmation sequences for line 291C10 and hit At5g14880 are shown.
Line ID 291C10
Locus At5g14880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4814693
Primers used for PCR SD41 - 8474, show primer details
   
Sequence Name 19-K103492-0022-291-C10C-SD41
GenBank Accession LN494725 [GenBank]
Sequencing primer gene specific primer, SD41: 5'-ATCGCATCTTTTGAAGTTTCTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103492-0022-291-C10C-SD41
ATACAAACCCATTCAATGGATCTCGAGCGTCTAAGCCCAAGAAATCCAGTTAAGGTATAA
AATTCTCAATATTCAATTCCATAATCCTTTCTTTCTTTTAGTTGCTTAAATTGGTAATGG
TTTTTGCAGAAAGAATCTTGGTGGACGGTTCTGACATTAGCTTACCAAAGCTTAGGTGTA
GTCTACGGAGATTTAGCAACTTCACCATTGTATGTCTACAAGAGCACTTTTGCTGAAGAC
ATAACACATTCTGAGACAAACGAAGAGATCTTTGGTGTTCTGTCTCTTATCTTCTGGACA
TTAACTTTAATCCCACTTGTTAAATACGTTTTTATTGTTCTTCGTGCTGATGATAATGGT
GAAGGTGGGACTTTTGCTCTCTACTCTTTATTATGTCGCCATGCTAGAATCAGCTCTTTG
CCCAATTTCCAGCTCGCTGATGAGGACCTTTCTGAGTATAAGAAAGAGTAAAAGAAAGCA
ATGCGTATACCAATCAGCAATTTAAAATGGCTTCAATTTTAAGCCATTGAGGACCTTTCG
GATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTC
   
Sequence Name 43-K103493-0022-291-C10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K103493-0022-291-C10C-8409
CCTTTACATGAATCCGAAGGTCCTCAATGGCTTAAATTGAAGCCATTTTAAATTGCTGAT
TGGTATACGCATTGCTTTCTTTTACTCTTTCTTATACTCAAAGGTCCTCATCACGACTGG
AAATTGGGCAAGAGCTGATTCTAGCAGGCGACTAATAAGAGTAAAGCAAAGTCCCACCTT
CCCATTAATCAGCCGAAAAAATAAACGTATTTACAAGTGGGATAAAGTTATGTCCGAAAT
AGAGACGAAACCAAATCTCTCTTGTCCAAAGTGTAGTCTTCGCAAATGCTCTGTAACTAC
ATGGTAATTGCTATCCCTAACACCCTAACTTTGGTACTAGTCAAACGTCCACCAGATCTT
CGCAAACCATTACCATTTAACACTAAAAAGAAGGATTAGGATTGAAATGAAATTATACTA
ACGGATTTCTGGGCTTAACCTGAGATCTTGAATGGGTTTGTACTAGATCAAGGATACATT
T

Last Updated on 06.12.2018 16:11