Sequences from confirmation amplicon


Confirmation sequences for line 291D01 and hit At5g49330 are shown.
Line ID 291D01
Locus At5g49330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19999570
Primers used for PCR RS01 - 8474, show primer details
   
Sequence Name 35-K016850-022-291-D01C-8409
GenBank Accession LN501622 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K016850-022-291-D01C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGAGTGGTTGGATGGTGAG
TTAGAACGGCTCTTGAGTAGTTGTGTCTGGGAATGCACTAGTGAAGAGGCTGTGATTGGA
GTAAATGATGAAAAGGTGTGTGAGAGTGGGGACAATAGTAGTTGTTGTGTTAATTTGTTT
GAAGAAGAACAAGGAAGCGAGACAAAGATTGGTCACGTAGGAATCACAGAGGTTGATCAT
GATATGACGGTGGAAAGAGAAAGAGAGGGAAGTTTTTTAAGTTCGAATTCAAATGAAAAT
AATGATAAAGATTGGTGGGTTGGTCTATGTAATTCTTCAGAAGTTGGGTTTGGGGTTGAT
GAGGAGTTGCTTGATTGGGAGTTTCAAGGTAATGTCACTTGTCAAAGTGATGATCTATGG
GATCTCTCAGATATTGGAGAGATAACATTGGAGTGATACTACCGAGCAAGTGGATTGTAC
ATTAGAAGGCGCGCGTAGAAATCCAATAAAACATCGATGTTTTGATTGTAAAGTCTAGTG
TTTTGTTTGGTTATAGTGGATCTTGTGGTTTTGTAG
   
Sequence Name 36-K016850-022-291-D01C-RS01
GenBank Accession LN502029 [GenBank]
Sequencing primer gene specific primer, RS01: 5'-CCAACAAGCTACTACAAAACCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K016850-022-291-D01C-rs01
GATCCACTATAACCAAACAAAACACTAGACTTTACAATCAAAACATCGATGTTTTATTGG
ATTTCTACGCGCGCCTTCTAATGTACAATCCACTTGCTCGGTAGTATCACTCCAATGTTA
TCTCTCCAATATCTGAGAGATCCCATAGATCATCACTTTGACAAGTGACATTACCTTGAA
ACTCCCAATCAAGCAACTCCTCATCAACCCCAAACCCAACTTCTGAAGAATTACATAGAC
CAACCCACCAATCTTTATCATTATTTTCATTTGAATTCGAACTTAAAAAACTTCCCTCTC
TTTCTCTTTCCACCGTCATATCATGATCAACCTCTGTGATTCCTACGTGACCAATCTTTG
TCTCGCTTCCTTGTTCTTCTTCAAACAAATTAACACAACAACTACTATTGTCCCCACTCT
CACACACCTTTTCATCATTTACTCCAATCACAGCCTCTTCACTAGTGCATTCCCAGACAC
AACTACTCAAGAGCCGTTCTAACTCACCATCCAACCACTCATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
Primers used for PCR 39xl - 3144, show primer details
   
Sequence Name 72-K119123-0022-291-D01C-39xl
GenBank Accession LN512810 [GenBank]
Sequencing primer gene specific primer, 39xl: 5'-ACTGTAACAATGAGTGGCAAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K119123-0022-291-D01C-39xl
TAGCTCCAATTTGAATTTTACTGTGCCTAAACTTCTAATCTTCTTCATTGAGTTTCGGTA
TTTTTAGTTGCAATAAAATTTAGAAACTTATTTACCAATAAATCAACTCATTTCTAGAAT
TAAGGTGTTTCTACGAAATCTGAATATATTCAGTGCTAACTCTTATAAAAAAATGAGGAA
CAGTCCAACCATTTTTAAAGAAAAATTAGTTACACATAACTAAACCATGCAATTTTTCTG
ACGTCATACATTTTGTTGTAGTATTGGTATTGAATTAATGAGAAGCCAACTTGTAAAGAG
TGTGTACACATAATTAATTGATTTGTCTAAATTTATGTTGTCTCACGTGTGTGCATGTGA
CTATTTTCTTATAGATGGTCACTTATTGCAACACATCTACCAGGAAGAACAGACAACGAA
ATTAAAAACTATTGGAACTCACATCTCAGCCGCAAAATCTATGCCTTCACTGCCGTTTCC
GGAGATGGACACAATCTACTCGTCAACGATGTAGTCTTGAAGAAATCTTGTTCATCGTCT
TCTGGAGCCAAGAACAATAACAAGACCAAGAAGAAGAAGAAGGGAAGGACTAGTAGGTCA
TCCATGAAGAAACACAAGCAAATGGTGACGGCCTCACAATGTTTCTCACAACCTAAGGAG
CTAGAGAGTGATTTCAGTGAGGGAGGGCAAAATGGTAATTTTGAAGGAGAGTCTTTGGAA
ACCCGGGTACCCTGTCCTCTCCAATGAAATGAACTTCCCTATATAGAGGAAGGGTCTTGC
GAAAGATAGTGGGATTGTG
   
Sequence Name 49-K119111-0022-291-D01C-35St
GenBank Accession LN507006 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K119111-0022-291-D01C-35St
AGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCNGGGTTT
CAAAGACTCTCCTTCAAAATTACCATTTTGCCCTCCCTCACTGAAATCACTCTCTAGCTC
CTTAGGTTGTGAGAAACATTGTGAGGCCGTCACCATTTGCTTGTGTTTCTTCATGGATGA
CCTACTAGTCCTTCCCTTCTTCTTCTTCTTGGTCTTGTTATTGTTCTTGGCTCCAGAAGA
CGATGAACAAGATTTCTTCAAGACTACATCGTTGACGAGTAGATTGTGTCCATCTCCGGA
AACGGCAGTGAAGGCATAGATTTTGCGGCTGAGATGTGAGTTCCAATAGTTTTTAATTTC
GTTGTCTGTTCTTCCTGGTAGATGTGTTGCAATAAGTGACCATCTATAAGAAAATAGTCA
CATGCACACACGTGAGACAACATAAATTTAGACAAATCAATTAATTATGTGTACACACTC
TTTACAAGTTGGCTTCTCATTAATTCAATACCAATACTACAACAAAATGTATGACGTCAG
AAAAATTGCATGGTTTAGTTATGTGTAACTAATTTTTCTTTAAAAATGGTTGGACTGTTC
CTCATTTTTTTATAAGAGTTAGCACTGAATATATTCAGATTTCGTAGAAACACCTTAATT
CTAGAAATGAGTTGATTTATTGGTAAATAAGTTTCTAAATTTTATTGCAACTAANAATAC
CGAAACTCAATGAAGAAGATTAGAAGTTTAGGCACAGTAAAATTCAAATTTGGAGCTNNT
AACATGCATTAACTCTATAGACTCA

Last Updated on 06.12.2018 16:11