Sequences from confirmation amplicon


Confirmation sequences for line 291F05 and hit At3g20540 are shown.
Line ID 291F05
Locus At3g20540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7172078
Primers used for PCR PF70 - 8474, show primer details
   
Sequence Name 03-K102864-0022-291-F05C-8409
GenBank Accession LN487439 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K102864-0022-291-F05C-8409
TCGGACTGAGCCATTTACATTGAATATAATATCATTGACTGTGATATCAATATCATTTCA
CCTGTCCATAATCAGCAACAATAAGTGAATTTCCAGGTGATGCTATGAAGGCCTGACGGA
TTTTGTACCGATCTTTCTCCAATGCAGGTTGGTTCTAGACAGAGAAAAGGAATACTTTAC
AAACAAATTCTAGATGTCCTATACCTCTATCTAAAAATTGACCTTGAGCACACCAATCCA
AAAATTAGCATATACTAGCTCTTCATACCACAACATCAAAAAAATTTACAAGGTTATCAG
TAGAGAAATTAGAGAAGCTTAGTTATTTGATTGCATCTCCATTCAAGCTAAC
   
Sequence Name 03-K102869-0022-291-F05C-PF70
GenBank Accession LN487440 [GenBank]
Sequencing primer gene specific primer, PF70: 5'-AGTTAGCTTGAATGGAGATGCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K102869-0022-291-F05C-PF70
TGATAACCTTGTAAATTTTTTTGATGTTGTGGTATGAAGAGCTCGTATATGCTAATTTTT
GGATTGGTGTGCTCAAGGTCAATTTTTAGATAGAGGTATAGGACATCTAGAATTTGTTTG
TAAAGTATTCCTTTTCTCTGTCTAGAACCAACCTGCATTGGAGAAAGATCGGTACAAAAT
CCGTCAGGCCTTCATAGCATCACCTGGAAATTCACTTATTGTTGCTGATTATGGACAGGT
GAAATGATATTGATATCACAGTCAATGATATTGATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATTGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11