Sequences from confirmation amplicon


Confirmation sequences for line 291F08 and hit At4g08930 are shown.
Line ID 291F08
Locus At4g08930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5730129
Primers used for PCR 10nt - 8474, show primer details
   
Sequence Name 72-K114626-0022-291-F08C-10nt
GenBank Accession LN512773 [GenBank]
Sequencing primer gene specific primer, 10nt: 5'-TCACTGTGAAGCTGAAACACTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K114626-0022-291-F08C-10nt
CCAAAGACTTGGAAGCCCATGCTCTAGCATTCATGGCTCCTTCCTGCAAATTGCTACTCA
TGCAAGGGTTCTTTAAGATACATGGTCACAGTATGTTCCAAGGGGTTCCCTAGTCTCATG
TTTGACACCCGTCCCCAAGTGAATTTCACGAACACAACCATTGTGGGGGAAATCAAGTGT
AGTAATCTCAACAAGACGAACACCGTGGCAAGCGTCAGATAAGTCTCTTGGCGAAGCAGA
TTCTCGGGTGATCTTCTTGCCCATGGGAACGGGCAATTTTCTGGCTCTGCGTGGAAATGA
GGAACTAATCTGTTTCTCTCAACCCATCTTTCATCCATCGTTTCAATTCCTACATGAATG
AATTTGTCCTGGTTAGTTTCATCTGCTGAAATCTTAATTGGTATATACAACCTATAACAG
GAGCACAATAGCAACCACAACAAGACTGAGGCACACAACAACACAAAGATATAGCCNAAG
TAACAACTCANATACCTTACCCGTAACATCAGTGTAGAAGGCAACAAGGGAATCAAGAAC
ATACATTTATGGATCAGCAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATATCCGC
   
Sequence Name 72-K114625-0022-291-F08C-8409
GenBank Accession LN512772 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K114625-0022-291-F08C-8409
ATTGCTGATCATAAATGTATGTTCTTGATTCCCTTGTTGCCTTCTACACTGATGTTACGG
GTAAGGTATTTGAGTTGTTACTTTGGCTATATCTTTGTGTTGTTGTGTGCCTCAGTCTTG
TTGTGGTTGCTATTGTGCTCCTGTTATAGGTTGTATATACCAATTAAGATTTCAGCAGAT
GAAACTAACCAGGACAAATTCATTCATGTAGGAATTGAAACGATGGATGAAAGATGGGTT
GAGAGAAACAGATTAGTTCCTCATTTCCACGCAGAGCCAGAAAATTGCCCGTTCCCATGG
GCAAGAAGATCACCCGAGAATCTGCTTCGCCAAGAGACTTATCTGACGCTTGCCACGGTG
TTCGTCTTGTTGAGATTACTACACTTGATTTCCCCCACAATGGTTGTGTTCGTGAAATTC
ACTTGGGGACGGGTGTCAAACATGAGACTAGGGAACCCCTTGGAACATACTGTGACCATG
TATCTNAAAGAACCTTGCATGAGTAGCAATTTGCAGGAAGGAGCCATGAATGCTAGAGCA
TGGGCTTCCAAGTCTTTGGCTACTGTCTCAATTGCAGAATCAAGCTCATCAAGCAGAAGT
GTTTCACTTCCACAGTG

Last Updated on 06.12.2018 16:11