Sequences from confirmation amplicon


Confirmation sequences for line 292C04 and hit At1g62120 are shown.
Line ID 292C04
Locus At1g62120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22960459
Primers used for PCR CQ41 - 8474, show primer details
   
Sequence Name 07-K105950-0022-292-C04C-CQ41
GenBank Accession LN489274 [GenBank]
Sequencing primer gene specific primer, CQ41: 5'-CAAAGCTCTTAACGTTCTTTACGG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105950-0022-292-C04C-CQ41
GATTGAATAATTCATGCCTGTAAAAGGTTAGGCTTGGCTGTGGACGATGTATGGGCAATG
TTCAAGAAGTGGCCAAACATTCTGACAAAGTCTGAGAAGAAGATAGAGAACTCCGTTGAA
ACATTTCTAGGCCTAGGATTCAGCAGAGATGAGTTTTTGATGATGGTCAAGCGATTCCCT
CAGTGCATTGGATATTCTACAGAGTTGATGAAGACGAAGACTGAGTTTCTGGTGACGGAG
ATGAATTGGCCACTAAAGGCTGTGGCTTCAATCCCTCAGGTACTCGGATACAGCCTAGAG
AAGAGGACTGTCCCAAGGTGTAATGTTATCAAAGTTCTCATCTCAAAAGGATTGCTCGAA
AGTGAACTCCCTCCAATATCCTCTGTTTTGACTCATACATACAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 23-K105951-0022-292-C04C-8409
GenBank Accession LN496558 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K105951-0022-292-C04C-8409
CATTTACAATTGAATATATCCTGTATGTATGAGTCAAAACAGAGGATATTGGAGGGAGTT
CACTTTCGAGCAATCCTTTTGAGATGAGAACTTTGATAACATTACACCTTGGGACAGTCC
TCTTCTCTAGGCTGTATCCGAGTACCTGAGGGATTGAAGCCACAGCCTTTAGTGGCCAAT
TCATCTCCGTCACCAGAAACTCAGTCTTCGTCTTCATCAACTCTGTAGAATATCCAATGC
ACTGAGGGAATCGCTTGACCATCATCAAAAACTCATCTCTGCTGAATCCTAGGCCTAGAA
ATGTTTCAACGGAGTTCTCTATCTTCTTCTCAGACTTTGTCAGAATGTTTGGCCACTTCT
TGAACATTGCCCATACATCGTCCACAGCCAAGCCTAACCTTTTACAGGCATTGAATTTAT
TTTCAATTCCTTTGTCGCTTAATCCGTAAAGAACGTAGNAAGCTTT

Last Updated on 06.12.2018 16:11