Sequences from confirmation amplicon


Confirmation sequences for line 292G03 and hit At5g47100 are shown.
Line ID 292G03
Locus At5g47100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19131546
Primers used for PCR JK13 - 8474, show primer details
   
Sequence Name 37-K020436-022-292-G03C-8409
GenBank Accession LN502454 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K020436-022-292-G03C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTCATATCAGTTAAC
AATTGATAAAAGAGAGATCTTTATGCTCATGCTCTTTGAGAATAAAGCAAAGAAGAGAAA
ACGAAACGTATAGTGCAAGTAAACTTACAAGCAGTCTCAGAAGCAAGCTTCACAGGGTTT
TCGTGATCGGGAAATTCTCTGGCAGCCGTGGAATGGAAACAACCCATTTACGGATGACAA
GAGTAAAACGAAGAAGAAGAAGCTTGTTCCGGAGGTTGATAAAATCGCCAAACTCTACTA
CAATTTCTGAGATTGTGAAAAAGCTTGATTTCTGATATTACTCACTCCCCATCATCATCA
TAAGCATCTTTAAAAACTACAATGATTCAATCAAAAACAAAACCCAACAAAAATTGAATT
AAAAGACGAACACGATAATTGAATAAGTATAAACCCTAGAAAAAATAAGCAGCAGCTATG
TGGCAATCGAATTCAGATTAACAAAATTTTGAATATTAAATCGGAAATTAAACAGGTAAT
GTAGGAGCGGCTCGAGAATCTCCGAACCCAGAAACTGAATGGTGAAATCGAAAAATTGAG
CACTTACCACCGAGAGAACGGGCTCGCCGGCTCGGT
   
Sequence Name 38-K020436-022-292-G03C-JK13
GenBank Accession LN502833 [GenBank]
Sequencing primer gene specific primer, JK13: 5'-AATCAAAATCCCTTCCTCTCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K020436-022-292-G03C-JK13
GCCCGTTCTCTCGGTGGTAAGTGCTCAATTTTTCGATTTCACCATTCAGTTTCTGGGTTC
GGAGATTCTCGAGCCGCTCCTACATTACCTGTTTAATTTCCGATTTAATATTCAAAATTT
TGTTAATCTGAATTCGATTGCCACATAGCTGCTGCTTATTTTTTCTAGGGTTTATACTTA
TTCAATTATCGTGTTCGTCTTTTAATTCAATTTTTGTTGGGTTTTGTTTTTGATTGAATC
ATTGTAGTTTTTAAAGATGCTTATGATGATGATGGGGAGTGAGTAATATCAGAAATCAAG
CTTTTTCACAATCTCAGAAATTGTAGTAGAGTTTGGCGATTTTATCAACCTCCGGAACAA
GCTTCTTCTTCTTCGTTTTACTCTTGTCATCCGTAAATGGGTTGTTTCCATTCCACGGCT
GCCAGAGAATTTCCCGATCACGAAAACCCTGTGAAGCTTGCTTCTGAGACTGCTTGTAAG
TTTACTTGCACTATACGTTTCGTTTTCTCTTCTTTGCTTTATTCTCAAAGAGCATGAGCA
TAAAGATCTCTCTTTTATCAATTGTTAACTGATATGAGCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCANCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT

Last Updated on 06.12.2018 16:11