Sequences from confirmation amplicon


Confirmation sequences for line 292G10 and hit At1g67600 are shown.
Line ID 292G10
Locus At1g67600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25337597
Primers used for PCR 10vi - 8474, show primer details
   
Sequence Name 19-K117806-0022-292-G10C-10vi
GenBank Accession LN494945 [GenBank]
Sequencing primer gene specific primer, 10vi: 5'-TTCAAATCCCATCTCCTTTCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117806-0022-292-G10C-10vi
ATTTCACACTAGTCTGTTTCTTTACCTAGACAGATATGAAAACGTACCAGGAGGTGAAGA
ATTTGATGAATTGTGCGATGGTGAAAGCTAAAACGGCGGAAATCAGAGGGTAATTTGTGA
AAATTGAGATATAGTGCGAGGAAGATGAAGCCACCGATTCCTCCATTGAATGATGGTGAT
CGATTGATCTCGTATCGTAGGGCTTTAATCGTCTGATAGCTCTTCTACAGGATAAAGAAC
ATACAGAGAAGACATAAAGTTGCGTGTAGAAATCTTTACCTTGAGACTCCTCTAATGGCT
TCTTCTTCTTCTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCACGGTTATAAT
   
Sequence Name 19-K117784-0022-292-G10C-8409
GenBank Accession LN494944 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117784-0022-292-G10C-8409
ATCCTGTAGAAGAAGAAGAAGCCATTAGAGGAGTCTCAAGGTAAAGATTTCTACACGCAA
CTTTATGTCTTCTCTGTATGTTCTTTATCCTGTAGAAGAGCTATCAGACGATTAAAGCCC
TACGATACGAGATCAATCGATCACCATCATTCAATGGAGGAATCGGTGGCTTCATCTTCC
TCGCACTATATCTCAATTTTCACAAATTACCCTCTGATTTCCGCCGTTTTAGCTTTCACC
ATCGCACAATTCATCAAATTCTTCACCTCCTGGTACGTTTTCATATCTGTCTAGGTTAAA
GAAACAGACTAGTTGTAGAAATTTGATTTTACAACTACTTGGTGTAGGTATAAAGAAAGG
AGAGGGGATTTGAA

Last Updated on 06.12.2018 16:11