Sequences from confirmation amplicon


Confirmation sequences for line 293C11 and hit At2g31800 are shown.
Line ID 293C11
Locus At2g31800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13521509
Primers used for PCR DL58 - 8474, show primer details
   
Sequence Name 03-K018514-022-293-C11C-DL58
GenBank Accession LN487280 [GenBank]
Sequencing primer gene specific primer, DL58: 5'-TGAACCAAATAAGCTCAAAGAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K018514-022-293-C11C-DL58
CAAATGATGTTACTGTAACTTAAAGTGTAAAACAAGTTCTGTTTTAAAGTTGATAAAGCT
TAGTACACTTACTCTCTCATCTCTTGGGGACAACTTTTGGACTTGGCTTTAAACGAAGGT
CTTCTTCCTTCTAAACACATTAGCTTCACTGCCTCTTCTGGGGGTTTAGGATGGAAAGGT
TGTACTCCTTCAATCATCTGAAATTTTTAAGATAAACTCATCAGAGAATTGATGCATGAG
ATTTGCTAGAGAAGCTTATTGGCCTCAATCAGTCAATGCTAGGAAAAACATAATCAGCTA
GAATGGTTGAGAAACAGAATGAAGTACTTTTAGAGAGCATATAGAACAGTGAGTGGAATA
AAACCAGACTAGATACAGCCATCAAGAAATGCCGAGGAGCACAATGTTTGACCATTAACG
TAACTAATAGGTACCGGATATAAGATGACACCAAAACGTATGAATCCATAAGGTACTACC
TCATATAATACGACACCAAAAGAGTAAGAATCCACACTCCTGTCAAATTGAAAATCAAAT
AACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
   
Sequence Name 04-K018514-022-293-C11C-8409
GenBank Accession LN487723 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K018514-022-293-C11C-8409
AATTTCCCGGACATGAAGCCATTTACAATTGGTTATTTGATTTTCAATTTGACAGGAGTG
TGGATTCTTACTCTTTTGGTGTCGTATTATATGAGGTAGTACCTTATGGATTCATACGTT
TTGGTGTCATCTTATATCCGGTACCTATTAGTTACGTTAATGGTCAAACATTGTGCTCCT
CGGCATTTCTTGATGGCTGTATCTAGTCTGGTTTTATTCCACTCACTGTTCTATATGCTC
TCTAAAAGTACTTCATTCTGTTTCTCAACCATTCTAGCTGATTATGTTTTTCCTAGCATT
GACTGATTGAGGCCAATAAGCTTCTCTAGCAAATCTCATGCATCAATTCTCTGATGAGTT
TATCTTAAAAATTTCAGATGATTGAAGGAGTACAACCTTTCCATCCTAAACCCCCAGAAG
AGGCAGTGAAGCTAATGTGTTTAGAAGGAAGAAGACCTTCGTTTAAAGCCAAGTCCAAAA
GTTGTCCCCAAGAGATGAGAGAGTAAGTGTACTAAGCTTTATCAACTTTAAAACAGAACT
TGTTTTACACTTTAAGTTACAGTAACATCATTTGCTTGGTCCTTGATCCTTTACCCAACC
TCTTTGAGC

Last Updated on 06.12.2018 16:11