Sequences from confirmation amplicon


Confirmation sequences for line 293G01 and hit At3g13570 are shown.
Line ID 293G01
Locus At3g13570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4430427
Primers used for PCR 21hw - 8474, show primer details
   
Sequence Name 42-K112689-0022-293-G01C-21hw
GenBank Accession LN504519 [GenBank]
Sequencing primer gene specific primer, 21hw: 5'-GGGTGAGGATGCTCTGTAGATAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K112689-0022-293-G01C-21hw
TATTTACATTACTTCATTAGACTATGTCTCTTTCTTATTGTTTTCTTTTGCTTCGTAAAC
CACCTTTTGTGAATCCTACATTCCATCGTATGCAGCTCATGAATTTGTTAGATTTATAAG
AATTTTCTGAAAGGTCTATGTCAAACATGTCCGGTTTTATATCCAATCTTCCCAAAATCA
CATTTGGAGCCTTGAAACGTTTGTTTTATCTACACTTAGGTTTTCAGCTTAAGTCTAGTA
CACCTAAAACTTCGCCCTCTTCGAGTCACTTTAGGGAAAGAATACAGTGATATGTTCCAT
GTTCGGTCACACTTCGAGTCTGTTTATTAAAGTTGTTGAGGTTTAACAGTGAATCTAGAG
AGTTGAAGAGACCAATGAAGTAATTAGAGTTCTTTGGAAGATGTTCTAAATGGTAGTGAA
GGTTGGAAGGAAGTTGGGTTTGAATTCAATTTGAAGAGATATCAAGTCTTGGAGAAGTCA
TCTTTATACTGAAACTTGCAAGGTTTGCAGGCAAGTGCTTGCTTTTTCATGTTTATAACG
ATGGCTTCTATTGCAGGAGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTC
   
Sequence Name 10-K112688-0022-293-G01C-8409
GenBank Accession LN490730 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K112688-0022-293-G01C-8409
GCATTTACATCTCCTGCATAGAAGCCATCGTTATAAACATGAAAAAGCAAGCACTTGCCT
GCAAACCTTGCAAGTTTCAGTATAAAGATGACTTCTCCAAGACTTGATATCTCTTCAAAT
TGAATTCAAACCCAACTTCCTTCCAACCTTCACTACCATTTAGAACATCTTCCAAAGAAC
TCTAATTACTTCATTGGTCTCTTCAACTCTCTAGATTCACTGTTAAACCTCAACAACTTT
AATAAACAGACTCGAAGTGTGACCGAACATGGAACATATCACTGTATTCTTTCCCTAAAG
TGACTCGAAGAGGGCGAAGTTTTAGGTGTACTAGACTTAAGCTGAAAACCTAAGTGTAGA
TAAAACAAACGTTTCAAGGCTCCAAATGTGATTTTGGGAAGATTGGATATAAAACCGGAC
ATGTTTGACATAGACCTTTCAGAAAATTCTTATAAATCTAACAAATTCATGAGCTGCATA
CGATGGAATGTAGGATTCACAAAAGGTGGTTTACGAAGCAAAAGAAAACAATAAGAAAGA
GACATAGTCTAATTGAAGTAATGTAAAATTATGCCCTGGATGTATAAACATTCAATGTCT
TATCTACAGA

Last Updated on 06.12.2018 16:11