Sequences from confirmation amplicon


Confirmation sequences for line 294A01 and hit At4g05130 are shown.
Line ID 294A01
Locus At4g05130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2638324
Primers used for PCR GG02 - 8474, show primer details
   
Sequence Name 92-K100901-0022-294-A01C-8409
GenBank Accession LN515174 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K100901-0022-294-A01C-8409
GCCTTTACATTGTGCATAGACAATATAATGTCCGATTCCACCGTGTCCTTTTGTTGTCAA
ATCCAACTGTTTTAAAGTTTTTTAGAGTCTTGAGTCCATAATAAAAATCCATGCAGTTCC
ATAAGAAAGATTAATTCTGAGAGTAAGACTTAAACTGATACTTACGACTATCAGCAAGAA
TGTGGATATCGTGAATAGGATGTAACCGGTAAGGATACGTTTCCTAGTACTGATTTTCGA
TTCGCGGTATGCGAGAATCATGATTGTTCCAAGGGCAATTGGTTGGTATATAAGTGTGAA
TACCCTTGAAGGATGATAATCCTACATGAAACACAAATTTTTCAAAATTTAAATTTAAAT
TGATAACTAAAACGAAATAGCAGAATTGAAACTCGAACCAAAACCGAAACCGAACCGGGA
AAACTTGATAGTAGTAATCTGCGATAGTGAGCATACTATTCCAAGAAAAGAGAGATCCAA
TTCCAAGAATACAACAAACAACCATTGCTTGATATTTCCCCTGTTTTTTAATACATACAT
AAACAAACAACTGAACAAGAATTTGATCAAAACACAATGAAAGATTGGGG
   
Sequence Name 92-K100907-0022-294-A01C-GG02
GenBank Accession LN515175 [GenBank]
Sequencing primer gene specific primer, GG02: 5'-AAAAACCCAAAATCTTTCATTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K100907-0022-294-A01C-GG02
TTTGTTATGTATGTATTAAAAAACAGGGGAAATATCAAGCAATGGTTGTTTGTTGTATTC
TTGGAATTGGATCTCTCTTTTCTTGGAATAGTATGCTCACTATCGCAGATTACTACTATC
AAGTTTTCCCGGTTCGGTTTCGGTTTTGGTTCGAGTTTCAATTCTGCTATTTCGTTTTAG
TTATCAATTTAAATTTAAATTTTGAAAAATTTGTGTTTCATGTAGGATTATCATCCTTCA
AGGGTATTCACACTTATATACCAACCAATTGCCCTTGGAACAATCATGATTCTCGCATAC
CGCGAATCGAAAATCAGTACTAGGAAACGTATCCTTACCGGTTACATCCTATTCACGATA
TCCACATTCTTGCTGATAGTCGTAAGTATCAGTTTAAGTCTTACTCTCAGAATTAATCTT
TCTTATGGAACTGCATGGATTTTTATTATGGACTCAAGACTCTAAAAAACTTTAAAACAG
TTGGATTTGACAACAAAAGGACACGGTGGAATCGGACATTATATTGTCTTATGCACAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATTC
Primers used for PCR GE65 - 8474, show primer details
   
Sequence Name 79-K100941-0022-294-A01C-8409
GenBank Accession LN513792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K100941-0022-294-A01C-8409
CGGACTGAGCCATTTACATTGTGCATAAGACAATATAATGTCCGATTCCACCGTGTCCTT
TTGTTGTCAATCCAACTGTTTTAAAGTTTTTTAGAGTCTTGAGTCCATAATAAAAATCCA
TGCAGTTCCATAAGAAAGATTAATTCTGAGAGTAAGACTTAAACTGATACTTACGACTAT
CAGCAAGAATGTGGATATCGTGAATAGGATGTAACCGGTAAGGATACGTTTCCTAGTACT
GATTTTCGATTCGCGGTATGCGAGAATCATGATTGTTCCAAGGGCAATTGGTTGGTATAT
AAGTGTGAATACCCTTGAAGGATGATAATCCTACATGAAACACAAATTTTTCAAAATTTA
AATTTAAATTGATAACTAAAACGAAATAGCAGAATTGAAACTCGAACCAAAACCGAAACC
GAACCGGGAAAACTTGATAGTAGTAATCTGCGATAGTGAGCATACTATTCCAAGAAAAGA
GAGATCCAATTCCAAGAATACAACAAACAACCATTGCTTGATATTTCCCCTGTTTTTTAA
TACATACATAAACAAACAACTGAACAAGAATTTGATCAAAACACAATGAAAGTTTTGGG
   
Sequence Name 79-K100949-0022-294-A01C-GE65
GenBank Accession LN513793 [GenBank]
Sequencing primer gene specific primer, GE65: 5'-AAAACCCAAAATCTTTCATTGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K100949-0022-294-A01C-GE65
GTTATGTATGTATTAAAAACAGGGGAAATATCAAGCAATGGTTGTTTGTTGTATTCTTGG
AATTGGATCTCTCTTTTCTTGGAATAGTATGCTCACTATCGCAGATTACTACTATCAAGT
TTTCCCGGTTCGGTTTCGGTTTTGGTTCGAGTTTCAATTCTGCTATTTCGTTTTAGTTAT
CAATTTAAATTTAAATTTTGAAAAATTTGTGTTTCATGTAGGATTATCATCCTTCAAGGG
TATTCACACTTATATACCAACCAATTGCCCTTGGAACAATCATGATTCTCGCATACCGCG
AATCGAAAATCAGTACTAGGAAACGTATCCTTACCGGTTACATCCTATTCACGATATCCA
CATTCTTGCTGATAGTCGTAAGTATCAGTTTAAGTCTTACTCTCAGAATTAATCTTTCTT
ATGGAACTGCATGGATTTTTATTATGGACTCAAGACTCTAAAAAACTTTAAAACAGTTGG
ATTTGACAACAAAAGGACACGGTGGAATCGGACATTATATTGTCTTATGCACAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT

Last Updated on 06.12.2018 16:11