Sequences from confirmation amplicon


Confirmation sequences for line 294B10 and hit At3g60950 are shown.
Line ID 294B10
Locus At3g60950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22526271
Primers used for PCR PF49 - 8474, show primer details
   
Sequence Name 53-K103693-0022-294-B10C-PF49
GenBank Accession LN508128 [GenBank]
Sequencing primer gene specific primer, PF49: 5'-GATAAGGGTCAGATTTTCCAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103693-0022-294-B10C-PF49
TTGCTTCACACTGTTACATAAGCTTCCTCTGTGGCTTAAGCTCAAAATCACTGAGAAAGA
AGAGAGATACATAGAATCGTCAAACTATATAGTGCTTTCTCAATACATATCATGACCTCG
CCAAGTAAATCCTATGGCCATACCAGAAGAGGAGAAAAGACAAAAGATTGACAGTGGCAG
GAAAAATTCTGTGAGTGAAAAAACGCAAAATGAAAAACAAGCAGAACACATGAAAGTACC
TTAAATCAACAGGTATACCACCAATGGGGAAAACAATTCTATGAGGCCACTGAAGCATGT
CTATAACGATAGTATCAACAGTGTCCTAACAGAAAGAGAAGCAAAAAGCTGTGTATAAGA
CCAAATGGGAACAACAACTAAAACTTTTGGAAACTGCTTGACCTACCAAGAGAGGAACTT
ACATCAATCATAGCAGATAGTCCAGGAATTGCTGTTAAGCTTCCACGGACAGCCTTCAGA
GTGTAATCAATTCTTGGTTTTGGCTGGTTGAGAACATTTAAATAGTTAATCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 69-K103694-0022-294-B10C-8409
GenBank Accession LN512186 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K103694-0022-294-B10C-8409
GCCATTTACAATTGATTAACTATTTAAATGTTCTCAACCAGCCAAAACCAAGAATTGATT
ACACTCTGAAGGCTGTCCGTGGAAGCTTAACAGCAATTCCTGGACTATCTGCTATGATTG
ATGTAAGTTCCTCTCTTGGTAGGTCAAGCAGTTTCCAAAAGTTTTAGTTGTTGTTCCCAT
TTGGTCTTATACACAGCTTTTTGCTTCTCTTTCTGTTAGGACACTGTTGATACTATCGTT
ATAGACATGCTTCAGTGGCCTCATAGAATTGTTTTCCCCATTGGTGGTATACCTGTTGAT
TTAAGGTACTTTCATGTGTTCTGCTTGTTTTTCATTTTGCGTTTTTTCACTCACAGAATT
TTTCCTGCCACTGTCAATCTTTTGTCTTTTCTCCTCTTCTGGTATGGCCATAGGATTTAC
TTGGCGAGGTCATGATATGTATTGAGAAAGCACTATATAGTTTGACGATTCTATGTATCT
CTCTTCTTTCTCAGTGATTTTGAGCTTAAGCCACAGAGGAAGCTTATAGTAACAGTAGTG
AAAGCAACTAACTTGAAGAATAAGGAATTGATTGGAAAATCT

Last Updated on 06.12.2018 16:11