Sequences from confirmation amplicon


Confirmation sequences for line 294E02 and hit At5g65330 are shown.
Line ID 294E02
Locus At5g65330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26111090
Primers used for PCR 08ou - 8474, show primer details
   
Sequence Name 57-K114954-0022-294-E02C-08ou
GenBank Accession LN509498 [GenBank]
Sequencing primer gene specific primer, 08ou: 5'-TATTAGTTGATTCAGACAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K114954-0022-294-E02C-08ou
TTTGTTTCTGCAAGTTTTTGTGAAAGATTTTTTATGGAAGTTCATATATATATGTCAATA
CTAACTGAAAAAAGTATCAACTTAAAAGTAAAAACAAAATCTCCACAAATGGATTTGTTT
TCTTAACAAAAAAAAAAAACAAAGGGAATTTGTTTGGCATTTATAAGGACCCTATAAAAT
TTAAAAAAAACCGTTACCAAAAAAATTCGAAGGCAAAAACAGTTTCAATCTCTCCCCAAT
CCGTTGGCAACTAAACTATGGACGGACTCTAAAGGAACCTCCAAAAAAACAATTTTTTTG
GAACAAATTTTGTTATCAAAAAATTCTGGATTTCCCGGGAAAACAAAAACGGCTAGAAAT
GTTGGGAAAAGGGGGCAAAAAAAATTTCTGTACAAAACGGCTCCCATCAAACCTGGAAAA
CTTGTCTGGAAAAAACCTAAGGTACCCAACCGCATTCCCAAAGGGGTTCGGGTTAAAATC
AAAAAAAAATTCAGGGGAACCCGTGTTTAAAAAATAAGAATGTTGAAAACCTTTTTTGTT
GTTAATAATTCCCCCCCCCAAACACTTGTTCGAATAAGGAAATATCCATTTGAAAAGGGC
TCCAGGTCCGGAAAATCTCCTGGGATCACCATGGATTATAAGGGTCAATAGGGAAAAAAA
AAAAAAGTATTTCCCATTTTTTTTCCATTTCAAAAAGGTAAATTTCCCC
   
Sequence Name 23-K114953-0022-294-E02C-8409
GenBank Accession LN496668 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114953-0022-294-E02C-8409
ATTTCAATTGAATAATCCTGATCAGAACATGTGTATGGTGAGTGAAATCATCAACAACAA
CAACGGTCTACAACATCCTAATCTCTCAAACACGGTTCCACATGAATTCTCTTCTGATTT
CAACCAGAACCCTTATGGTAATGCGGTCGGTAACATTAGCTTCTCTCAAGACATGTTTTC
AAGCTATGATGCGAGCAGTCTGCTACAGACATCTTCTCTGCCACCTCTCCACAACATTCC
TAGCAGTTATTGTTTTCCTGGCAACTCAAGACTTCTCTGATAACAAAATCTGTCCAAAAG
AATTTGTTTTTTTGAAGCTTCATTAAGAGTCAGTCAATAGTTAAGTAGCAAGCGGATCGG
AGAGAGATTGAAGCTGTTTCTGCTTCCGATTTCTTCTGTTACTGTTTTTTTTAATATTTT
ATAGTGTCATTATAACTGCAAAACAAATCCATTTGTTTTTTTTTTTTGTTAAAAAAACAA
ATCCTTTTGGGGAAATTTTGTTTTAACTTTAAATTGAAAACTTTTTCCGTTTAGTTTGGA
CATATATTTTTAAACTTCCAAAAAAAATTTTTCAAAAAACCTTGCAGGAAACAAATTATA
TTCTAAAACCCTAAATTCTGGCAACTAAATACCCTTCTGGTCGAAACCAACAAAAAA

Last Updated on 06.12.2018 16:11