Sequences from confirmation amplicon


Confirmation sequences for line 294E10 and hit At4g14910 are shown.
Line ID 294E10
Locus At4g14910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8528549
Primers used for PCR 07jr - 8474, show primer details
   
Sequence Name 30-K113214-0022-294-E10C-8409
GenBank Accession LN499798 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K113214-0022-294-E10C-8409
TTTGCTTCTTTCAATTGGATTTTGTTATCTGAATCTTTGCAATAAATAGTGGATGATGAA
TGAGATGTGTAGTGTGTGTCAAAGTCAAACCTTGGTATTGTCCCACCGCGGCGTGGATCA
GACTCTGTTGCTTGTCGGAGAGCTCTTGCAAAGGCCTTAAAGGTCGCTTCTATTATGTGA
TGCGAGTTTTTACCAGCAAGCTGTCATAATAAATAGAAATGAATCAAATCTTATCAAGAA
AGGTTTGTCAACAGATGAAACATTAACGTTCTGTTATTGTCCTCGTATGCTTTAAGAAAG
ATTGGAATCCTAATACCTGTCGGATGTGAAGAGTCATACCAGAAGTATTCACCAATGACT
GGAAGAAGTGTTCCACCAACTAAATGGGAAAAGAGTCATTTAGTGATTTTAACAAACAAA
GAAAACACTAGAAAAACCGAAAAACTGGATACTAAAATACTAAATGTGAAGAACCTGAGT
GTCGTATGTTCC
   
Sequence Name 30-K113215-0022-294-E10C-07jr
GenBank Accession LN499799 [GenBank]
Sequencing primer gene specific primer, 07jr: 5'-AGGAACATACGACACTCAGGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K113215-0022-294-E10C-07jr
AGTTTTCGGTTTTTCAGTGTTTTCTTTGTTTGTTAAATCACTAAATGACTCTTTTCCCAT
TTAGTTGGTGGAACACTTCTTCCAGTCATTGGTGAATACTTCTGGTATGACTCTTCACAT
CCGACAGGTATTAGGATTCCAATCTTTCTTAAAGCATACGAGGACAATAACAGAACGTTA
ATGTTTCATCTGTTGACAAACCTTTCTTGATAAGATTTGATTCATTTCTATTTATTATGA
CAGCTTGCTGGTAAAAACTCGCATCACATAATAGAAGCGACCTTTAAGGCCTTTGCAAGA
GCTCTCCGACAAGCAACAGAGTCTGATCCACGCCGCGGTGGGACAATACCAAGGTTTGAC
TTTGACACACACTACACATCTCATTCATCATCCACTATTTATTTGCAAAGATTCATGATA
ACAAAATCCAATTTGGAAAGAAGCAAATTATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCACGATTATT

Last Updated on 06.12.2018 16:11