Sequences from confirmation amplicon


Confirmation sequences for line 294G01 and hit At1g16390 are shown.
Line ID 294G01
Locus At1g16390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5603136
Primers used for PCR UF10 - 8474, show primer details
   
Sequence Name 29-K021852-022-294-G01C-8409
GenBank Accession LN499107 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K021852-022-294-G01C-8409
TGAGCCATTTACAATTGAAATATCCTGACAACTCGGACCGAGTCGACTCAGTATGCAACA
CATCCTCCTCCAACCTCTGCACCTTACCCAACCAAACCTGGTCTTGGGACTTGAACCCAC
ACGTTTCAATCATTTCCGAGTGGGGGCTACAATGCGCCGGCTCGTTCCTCAAGGGATTTC
CTGCAAGCTCATTCTTCCTCGGCTGTCTAATCGGCGGCTTAGCTCTCTCTACACTCGCCG
ACTCATCGCTCGGTCGTAAGAACATGCTCTTGTTGTCGTGTCTCATAATGTCTCTCTCTT
CCATGTTAACAGCTTTCTCAACGAGCATTTGGGTTTATGCGTTTTTGAGATTCTTGAACG
GGTGTGGCCGAGCCACGATCGGGACTTGCGCGCTTGTTCTATCAACGGAGTTAAGTCG
   
Sequence Name 30-K021852-022-294-G01C-UF10
GenBank Accession LN499544 [GenBank]
Sequencing primer gene specific primer, UF10: 5'-ACCGACTAACTCCGTTGATAGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K021852-022-294-G01C-UF10
GGCCCCCCGTTCAGATCTCAAACGCATAAACCCAAATGCTCGTTGAGAAAGCTGTTAACA
TGGAAGAGAGAGACATTATGAGACACGACAACAAGAGCATGTTCTTACGACCGAGCGATG
AGTCGGCGAGTGTAGAGAGAGCTAAGCCGCCGATTAGACAGCCGAGGAAGAATGAGCTTG
CAGGAAATCCCTTGAGGAACGAGCCGGCGCATTGTAGCCCCCACTCGGAAATGATTGAAA
CGTGTGGGTTCAAGTCCCAAGACCAGGTTTGGTTGGGTAAGGTGCAGAGGTTGGAGGAGG
ATGTGTTGCATACTGAGTCGACTCGGTCCGAGTTGTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11