Sequences from confirmation amplicon


Confirmation sequences for line 294G06 and hit At3g02740 are shown.
Line ID 294G06
Locus At3g02740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:591316
Primers used for PCR CQ47 - 8474, show primer details
   
Sequence Name 31-K112307-0022-294-G06C-CQ47
GenBank Accession LN500225 [GenBank]
Sequencing primer gene specific primer, CQ47: 5'-TTTACCAGAACCTGTAATTTTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112307-0022-294-G06C-CQ47
TTTTTTGTCTTTTTTAATGTGGAATCTGAAGAAAAACAAAGCTTTTGAATCTATTGAGAT
TCTCAACTCTTTGATTGGTTCTTGACTCATTATGAAATGGTCTTCTTCTTGTGTCTTCTA
CGGTAAAATGTTGATTCTTGTTCTTTATGTTTGTTGTTTTTAATTTCAGTTTATACTTCG
CTAAAATCGGACTCGGAACTCCATCAAGAGACTTCCATGTTCAAGTTGATACAGGAAGTG
ATATCTTATGGGTGAATTGTGCTGGTTGCATCAGATGTCCTAGGAAAAGTGATCTGGTAT
ATACCATCAATTCTATCCCTCCAAAAAAAAAAACAAAACTCTAATTTACTTTATGTTTTC
ATTTTCAAATTTCCATTATCTTTCAGGTAAAGCTAACCCCTTAGAATGGGAATGCTTCGC
CCACTGCAAAATCTGTTTCGGGCAGAATATATCCAATTGAAAAGGGCTTCATGTCCGGAA
AATCTACTTGGATCACCATTGAGTATAATGGTCAATATGGAAAAAAAAAAAAAGTATTTC
CCATTTTTTTTTCATTTCAAAAATGTAAATTTCCCC
   
Sequence Name 15-K112306-0022-294-G06C-8409
GenBank Accession LN493036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112306-0022-294-G06C-8409
CATTTACATTGAATATATCCTGCACGAAACAGATTTTGCAGTGGACGAAGCATCCACATC
ATAAGGTGTCAGCTCTACCTGAAAGATAATGAAACTATGAAACTGAAAACATAATGTAAC
TTAGAGTTCTGTTTTTTTTTTTGGAGGGATAAAATTGAGGGTATATCCCAAATCACTTTT
CCTAGGACATCTAAGGCACCCAGCACAATTCCCCCATAAAATATCACTTCCGGTATCAAC
TTGAACAGGGAATTCTCTGGAGGGATTCCCAAGCCCAATTTTACCGAATTATAACCGGAA
TTTAAAACCACCAAACTTAAAGAACAAAAATCACCTTTTTCCCGTAAAAAACCCAAAAAA
AAAACCTTTTCATAAGGAGTCAAAACCCAATCAAAGAGTTGAAAATCCCAATAAATCCAA
AACCTTTGTTTTTTCTTCAAATCCCACTTTTAAAAAAAACAAAAAAAACCCAACTTTTTG
TTAAAAAATTCCCAAAATTCCGGGTTCCGGGTAAAA

Last Updated on 06.12.2018 16:11