Sequences from confirmation amplicon


Confirmation sequences for line 294G07 and hit At2g28320 are shown.
Line ID 294G07
Locus At2g28320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12098597
Primers used for PCR 45fr - 8474, show primer details
   
Sequence Name 22-K111563-0022-294-G07C-45fr
GenBank Accession LN496172 [GenBank]
Sequencing primer gene specific primer, 45fr: 5'-TTCTTTCCCACACTTTGCTTAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K111563-0022-294-G07C-45fr
CCACCAAAGTCAGGTTGAATGCATTGGACAGACAAATAATAAAACTAAATAGAGGTAATC
TTTTACGCCAACCTTGGATATGTAGGGAATGAGCTTGAACCTTGAATTCCTATATGCGTC
ATCACCATTAACAAAGCTAACTAGCAAAGGATGCTCTTCAATAGGAGTGCTCATCATGTA
ATAAAGCACAAGGCTGTACGTTGTTGATCCAGGAACCTATTATTCCATCGAACAACTAAT
TGATTCTTTTACGGACACATGGAAAAGCTGAATAAAAAAACAATGAAAAAGATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 06-K111556-0022-294-G07C-8409
GenBank Accession LN488873 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K111556-0022-294-G07C-8409
TCTTTTTCTTGTTTTTTTTTCAGCTTTTCCATGTGTCCGTAAAAGAATCAATTAGTTGTT
CGATGGAATAATAGGTTCCTGGATCAACAACGTACAGCCTTGTGCTTTATTACATGATGA
GCACTCCTATTGAAGAGCATCCTTTGCTAGTTAGCTTTGTTAATGGTGATGACGCATATA
GGAATTCAAGGTTCAAGCTCATTCCCTACATATCCAAGGTTGGCGTAAAAGATTACCTCT
ATTTAGTTTTATTATTTGTCTGTCCAATGCATTCATACCTGACTTTTGGTTTGGCGATAT
TTTCAGGGCTCTTGGATAGTTAAGCAAAGTGTGGGAAAG

Last Updated on 06.12.2018 16:11