Sequences from confirmation amplicon


Confirmation sequences for line 295A02 and hit At3g24506 are shown.
Line ID 295A02
Locus At3g24506 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8929307
Primers used for PCR 05cz - 8474, show primer details
   
Sequence Name 22-K109029-0022-295-A02C-05cz
GenBank Accession LN496147 [GenBank]
Sequencing primer gene specific primer, 05cz: 5'-GTATGATCTACACAGGCACAGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K109029-0022-295-A02C-05cz
TCTCTCAGTATATTTGTGACTTAATTTCAAATTTCTGTATCAAAATTGACATTACAAGCA
CAGTAGCAATGTGAAATTTCGACGAATCTGTATGCAATTTTCTATAAGGAATCGAAGCAA
AGAGAGAGACTAACGATAGGGAAATCGAGAGGATTGCGACGAGTGAGCTTCTCTTCCTCA
GGAGCCATAGCCACCACCTGTAACTGCTTCCTTGTCTTGCGGCTTAGAGCGAAGCGTTGA
ACGGCGATGAAAGAGCACGAGAAGCCATGGTTGATTCGAGAGAAAGGGGAAAAAATAGAC
GGAGCAGCTCTGTTTAACGACGATGAAGATAGTGGCGGAGAGAGAAGTGGCCACGGCGGC
GAAGGCGATGCCGATATAGAAAGCGGCGTCATAGCTCCGCCTCGTGTTCTTCGATGGTTC
CTATATTTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
T
   
Sequence Name 06-K109028-0022-295-A02C-8409
GenBank Accession LN488849 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K109028-0022-295-A02C-8409
AGGACCATCGAAGAACACGAGGCGGAGCTATGACGCCGCTTTCTATATCGGCATCGCCTT
CGCCGCCGTGGCCACTTCTCTCTCCGCCACTATCTTCATCGTCGTTAAACAGAGCTGCTC
CGTCTATTTTTTCCCCTTTCTCTCGAATCAACCATGGCTTCTCGTGCTCTTTCATCGCCG
TTCAACGCTTCGCTCTAAGCCGCAAGACAAGGAAGCAGTTACAGGTGGTGGCTATGGCTC
CTGAGGAAGAGAAGCTCACTCGTCGCAATCCTCTCGATTTCCCTATCGTTAGTCTCTCTC
TTTGCTTCGATTCCTTATAGAAAATTGCATACAGATTCGTCGAAATTTCACATTGCTACT
GTGCTTGTAATGTCAATTTTGATACAGAAATTTGAAATTAAGTCACAAATATACTTGAGA
GATTGTATTTGGAATCTGAAATTTTCATGAGCAGATTCGTGTCCTGTGCCTGTGTAGATC
ATA

Last Updated on 06.12.2018 16:11