Sequences from confirmation amplicon


Confirmation sequences for line 295B01 and hit At2g03050 are shown.
Line ID 295B01
Locus At2g03050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:900217
Primers used for PCR 06ce - 8474, show primer details
   
Sequence Name 25-K108417-0022-295-B01C-06ce
GenBank Accession LN497477 [GenBank]
Sequencing primer gene specific primer, 06ce: 5'-CCTTAACGTAGATCCTCACAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K108417-0022-295-B01C-06ce
CGTTCGCTCCATCTCCTCCGTCGTCTCCGTCGAGACTCTACTCTCTTCCACCGGACTCTC
AAGACCCGCCGTTGGTCGTATCCTCGACATGTTTCCAGATTTGTTAACTTCTGATCCTGA
ATCTGAAATCTTACCAGTTCTTCGCTTCTTATCCAATGAGATTTCAATTTCAGAGCAAGA
CATACCTAAATCGATCTCTCGTTGTCCTCGATTGCTAATTTCATCTGTAGATTATCAGCT
TCGTCCTGCATTAACGTTCCTCAAAACCCTAGGATTCGTGGGTCGCGACACGATTACGTC
ACGGAACACGGTGTTGCTTGTATCGAATGTGGAACGAACCCTAATCCCCAAAATTGAGTA
TCTTGAAGAAGGGTTAGGGTTTACAAGAGAAGAAGTAGCGAAGATGGTTGTGAGATCTCC
GGCGTTGTTGACTTATAGTGTTGATAACAATTTAGTTCCTAAAGTTGAGTTTTTTATTGA
GGAGATGCGTGGAGATGTTAAGGAGCTGAAACGTTTCCCTCAGTATCTTTCGTTTAGCTT
GCAGAGGAAGATAAAGCCCTGACATTCCATTGATGGCTACATCCCTCACTATTGTGAT
   
Sequence Name 09-K108416-0022-295-B01C-8409
GenBank Accession LN490214 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K108416-0022-295-B01C-8409
TTGAATATATCCTGAGGGAAACGTTTGAACAACAACCATCACCATTTTGATGAATATAGC
CATCAATTGAATCTCATGGCTTTATCTTCCTCTCCAAGCTAAACGAAAAATACTGAGGGA
AACGTTTCAGCTCCTTAACATCTCCACGCATCTCCTCAATAAAAAACTCAACTTTAGGAA
CTAAATTGTTATCAACACTATAAGTCAACAACGCCGGAGATCTCACAACCATCTTCGCTA
CTTCTTCTCTTGTAAACCCTAACCCTTCTTCAAGATACTCAATTTTGGGGATTAGGGTTC
GTTCCACATTCGATACAAGCAACACCGTGTTCCGTGACGTAATCGTGTCGCGACCCACGA
ATCCTAGGGTTTTGAGGAACGTTCATGCAGGACGAAGCTGATAATCTACAGATGAAATTA
CCCCTCCAGGACAACGAGAGATCCATTTAGGTATGTTTTGCTCCCAAATTGAACTCCCCT
TGGAT

Last Updated on 06.12.2018 16:11