Sequences from confirmation amplicon


Confirmation sequences for line 295C07 and hit At1g61820 are shown.
Line ID 295C07
Locus At1g61820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22836636
Primers used for PCR ND13 - 8474, show primer details
   
Sequence Name 11-K032004-022-295-C07C-8409
GenBank Accession LN490999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K032004-022-295-C07C-8409
TTACATTGAATATATCCTGACAATATTAAATTAAGAAGTAGGGAGAGTAAATATTACCTT
TTTTAATAAGAGCGTCGATCAAATTGTTGTAATACTTTATTCCCTTATAATTAATAACTC
CAAATCTTCCATCTGTATTTTTATTACATAAGTTAACAGTTTGTTTTATTTTTATCTATA
TATCAAAGGAAAGGTACACACTTTTAACATATTGTTTCCGGTTTAGATATCTGAACCAAT
CAATTGATGGTAGAAGTTTAATTTCAAACCAAATTTAATTATTACAGTCATGATTAAGAA
GATAAAGACTTACTAGGTAAGACTCTAGACCAGGAAATAGATAATCTGTAACTATTGACT
CCAAGAAAATTCATTGATTGGATATCTTCCTGTAATCATATATAGTAGATAGATGTCAAT
ATAAAAAAGAATTAAAAACAACTAAAGTAAATAGTTTATTCAGGTAAAAGGAACAAAATA
TTATAATAATGTATTTATTTTACCATATATCGATGATATTGGTCCGTAGCTATGTCTCCA
TTGCTTCCATCAACTATTTTTCCCTGA
   
Sequence Name 12-K032004-022-295-C07C-ND13
GenBank Accession LN491458 [GenBank]
Sequencing primer gene specific primer, ND13: 5'-TTCAGGGAAAATAGTTGATGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K032004-022-295-C07C-ND13
GACATATCATCGATATATGGTAAAATAAATACATTATTATAATATTTTGTTCCTTTTACC
TGAATAAACTATTTACTTTAGTTGTTTTTAATTCTTTTTTATATTGACATCTATCTACTA
TATATGATTACAGGAAGATATCCAATCAATGAATTTTCTTGGAGTCAATAGTTACAGATT
ATCTATTTCCTGGTCTAGAGTCTTACCTAGTAAGTCTTTATCTTCTTAATCATGACTGTA
ATAATTAAATTTGGTTTGAAATTAAACTTCTACCATCAATTGATTGGTTCAGATATCTAA
ACCGGAAACAATATGTTAAAAGTGTGTACCTTTCCTTTGATATATAGATAAAAATAAAAC
AAACTGTTAACTTATGTAATAAAAATACAGATGGAAGATTTGGAGTTATTAATTATAAGG
GAATAAAGTATTACAACAATTTGATCGACGCTCTTATTAAAAAAGGTAATATTTACTCTC
CCTACTTCTTAATTTAATATTTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR FP84 - 8474, show primer details
   
Sequence Name 43-K035337-022-295-C07C-8409
GenBank Accession LN504753 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K035337-022-295-C07C-8409
GGAATGAAGCCATTTACATTGAATATATCCCGGACAGAAATTTTAACTTCAAAAGATTGC
TATTTTTCATGTAAATGTTTACAGGGATTACACCATTTGTGACGTTGAACCACTTCGACT
ATCCTCAAGAACTCGAGAACCGGTTCAAGAGTTGGTTAAGCTCCGAGATGCAGTGAGTTG
ATAAAATTAAGTCTCTATCTATTTTACCATAACTAAAACTGATTCATTCACTGTTTATAA
TTCAGGAAAGATTTCGGGTACTTAGCTGATATATGTTTCAAACATTTTGGAGACCGAGTT
AAACACTGGATCACGATTAACGAACCAAACCAACATA
   
Sequence Name 43-K035337-022-295-C07C-FP84
GenBank Accession LN504754 [GenBank]
Sequencing primer gene specific primer, FP84: 5'-GCTTATATGTTGGTTTGGTTCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K035337-022-295-C07C-FP84
ACTCGGTCCCAAATGTTTGAACATATATCAGCTAAGTACCCGAAATCTTTCCTGAATTAT
AAACAGTGAATGAATCAGTTTTAGTTATGGTAAAATAGATAGAGACTTAATTTTATCAAC
TCACTGCATCTCGGAGCTTAACCAACTCTTGAACCGGTTCTCGAGTTCTTGAGGATAGTC
GAAGTGGTTCAACGTCACAAATGGTGTAATCCCTGTAAACATTTACATGAAAAATAGCAA
TCTTTTGAAGTTAAAATTTCTGTCCGGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11