Sequences from confirmation amplicon


Confirmation sequences for line 295C10 and hit At5g42800 are shown.
Line ID 295C10
Locus At5g42800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17166067
Primers used for PCR ZZ80 - 8474, show primer details
   
Sequence Name 33-K021972-022-295-C10C-8409
GenBank Accession LN500822 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K021972-022-295-C10C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAGACTAAAGAGATGTGTGGTACGTA
ACAATCTGGTAAGTTGATTTTATTATTTCCTTAGGTGTGTATTTTGTTGTTGTACCGGTG
GGTGAAATACGTTGACTTCGATTTGTTTGGTGAGACGTGTGGGTGACTTCAGTTCAGTTT
GTTTTTGTTTTTGTTCCCCACCCACTTTTACGAAATAAATCAAAATTTGTCTAAACAAAT
TAACATTTTGATAGAATATATACAGAAATACATTTTACTTAAGTAATATTTTAATTTTAA
TTGAAATCTAAAAATCAGACACCTAAAATAATTATAAATCTTGGAAAAGCTTAAATGAAA
AATATGTTTATATTTTAGATTTATGAATTAGGAGTTTCTTATTACAAAATTGTAAAATTA
TAAAACATATTAATTTATAAATATGACTTCTAGATTTATTGCAATCTAAATATTATAAGC
ATTTACATAAAATACATAAACTAAAACTTTAAAAGATGTAATTA
   
Sequence Name 34-K021972-022-295-C10C-ZZ80
GenBank Accession LN501246 [GenBank]
Sequencing primer gene specific primer, ZZ80: 5'-ATAATGAAAAAGCTGACATGGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K021972-022-295-C10C-ZZ80
TGGATAATTACATCTTTTAAAGTTTTAGTTTATGTATTTTATGTAAATGCTTATAATATT
TAGATTGCAATAAATCTAGAAGTCATATTTATAAATTAATATGTTTTATAATTTTACAAT
TTTGTAATAAGAAACTCCTAATTCATAAATCTAAAATATAAACATATTTTTCATTTAAGC
TTTTCCAAGATTTATAATTATTTTAGGTGTCTGATTTTTAGATTTCAATTAAAATTAAAA
TATTACTTAAGTAAAATGTATTTCTGTATATATTCTATCAAAATGTTAATTTGTTTAGAC
AAATTTTGATTTATTTCGTAAAAGTGGGTGGGGAACAAAAACAAAAACAAACTGAACTGA
AGTCACCCACACGTCTCACCAAACAAATCGAAGTCAACGTATTTCACCCACCGGTACAAC
AACAAAATACACACCTAAGGAAATAATAAAATCAACTTACCAGATTGTTACGTACCACAC
ATCTCTTTAGTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGACAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACG

Last Updated on 06.12.2018 16:11