Sequences from confirmation amplicon


Confirmation sequences for line 296A02 and hit At1g45332 are shown.
Line ID 296A02
Locus At1g45332 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17175063
Primers used for PCR 17uv - 8474, show primer details
   
Sequence Name 34-K117403-0022-296-A02C-17uv
GenBank Accession LN501575 [GenBank]
Sequencing primer gene specific primer, 17uv: 5'-GGTTTTAGCTTGCCTTAGTCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117403-0022-296-A02C-17uv
ACGTTTAGATATTATTACTCTTTTACTAGCATTTTGTTACTTTAACTTTTGTGGTCTGCT
GTTTATATGTGCAGGAAGCTATTCGTAGAGCTACCATAGCACAAACGTTTGTTCCTGTAT
TTATGGGTAGTGCATTCAAAAACAAGGTAATTGATTCTTGGAATCCAAAGATGATATATA
TGCGCAAGTTTGGATGACATATTCACTTGCGTTTCTCCTTGTCCTCTTGTGTCTGTATTA
AAAAGTTTTCAACAACTTTTTTTAATCTGCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 10-K117402-0022-296-A02C-8409
GenBank Accession LN490842 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117402-0022-296-A02C-8409
AGATAAAAAAAGTTGTTGAAAACTTTTTAATACAGACACAAGAGGACAAGGAGAAACGCA
AGTGAATATGTCATCCAAACTTGCGCATATATATCATCTTTGGATTCCAAGAATCAATTA
CCTTGTTTTTGAATGCACTACCCATAAATACAGGAACAAACGTTTGTGCTATGGTAGCTC
TACGAATAGCTTCCTGCACATATAAACAGCAGACCACAAAAGTTAAAGTAACAAAATGCT
AGTAAAAGAGTAATAATATCTAAACGTGGCAATTCTTGGACGCTAGAAATGAAAGATAAC
ACTAAGATTTTAAGACAATGACTAAGGCAGCCATAAAACC

Last Updated on 06.12.2018 16:11