Sequences from confirmation amplicon


Confirmation sequences for line 296F07 and hit At2g41490 are shown.
Line ID 296F07
Locus At2g41490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17301876
Primers used for PCR SR42 - 8474, show primer details
   
Sequence Name 45-K103582-0022-296-F07C-SR42
GenBank Accession LN505503 [GenBank]
Sequencing primer gene specific primer, SR42: 5'-AATATTATCGCCACGATCAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K103582-0022-296-F07C-SR42
GCCTGAAACAAAGAGGATGAAGAAATTTTAATCAAACTAATCTTAATCTTTGGAAAATCA
GAGATTGATAAAAACTCACACTTTAATATCTCCTTGAGGCGTGCCTCTTTTGTTGATATC
AAAACCAAACATGTTACGTCTCAGAACGTATCTGGCAGTAACAGGAATCATTTTGAGAGT
GACGAAAAACCCAACCAAGCTAAGCCCCGCGTTAATTAGAATCGATCGCTTGAGCTCATT
ATCAACCTTGTAGTGAAAACAGAGAAGGTATAGATAGAGCGAACAGAGAAGAGTAGAGAT
CACGAAGATCACTCCTAATTTCGGCGGCGCGAGACGAAATTCCTCACCGGAAGCAGAAAC
CGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 61-K103583-0022-296-F07C-8409
GenBank Accession LN510497 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K103583-0022-296-F07C-8409
GGTGAGGAATTTCGTCTCGCGCCGCCGAAATTAGGAGTGATCTTCGTGATCTCTACTCTT
CTCTGTTCGCTCTATCTATACCTTCTCTGTTTTCACTACAAGGTTGATAATGAGCTCAAG
CGATCGATTCTAATTAACGCGGGGCTTAGCTTGGTTGGGTTTTTCGTCACTCTCAAAATG
ATTCCTGTTACTGCCAGATACGTTCTGAGACGTAACATGTTTGGTTTTGATATCAACAAA
AGAGGCACGCCTCAAGGAGATATTAAAGTGTGAGTTTTTATCAATCTCTGATTTTCCAAA
GATTAAGATTAGTTTGATTAAAATTTCTTCATCCTCTTTGTTTTCAGGCCAGAGTCATTG
GGTATTGTTGTTGGTATTGTCTTTTTGATCG

Last Updated on 06.12.2018 16:11