Sequences from confirmation amplicon


Confirmation sequences for line 296G02 and hit At3g24430 are shown.
Line ID 296G02
Locus At3g24430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8872208
Primers used for PCR JS01 - 8474, show primer details
   
Sequence Name 37-K020889-022-296-G02C-8409
GenBank Accession LN502455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K020889-022-296-G02C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAAGCTTTGAAGCCATTTTATCACAGAC
CGCTAGTTTTTTTTGCCACTCTCTTCGTCTCTCTCTTGTAGCAATGCCGCTTCTTCATCC
ACAGTCGTTGCGGCATCCTTCTTTCGAGATTCAGACCCAAAGAAGAAGCAATTCCACAAC
AAGATTGCTTCTTTCTCACAAGTTTCTCCATTCTCAAGCTTCCATTATCTCAATCTCCAG
GACAAGAATCCTCAAACGGGTCTCTCAGAATCTCTCTGTAGCTAAAGCTGCTTCAGCTCA
AGGTTATAAAGTTTGTTCCTTTGTCTCCGCCTGAAAAATTGTTAACGTTATACGCGAAGG
AGACTAAGCAAGTTCTGATATTTTTTAACTTATGCATTTTGGAAAACAGCTAGTAGTAGT
GTTGGTGAGAGTGTTGCTCAAACATCAGAAAAAGATGTGTTGAAGGCTCTGTCTCAGATT
ATTGATCCTGATTTTGGGACAGATATTGTTTCTTGTGGTTTTGTGAAAGATTT
   
Sequence Name 38-K020889-022-296-G02C-JS01
GenBank Accession LN502834 [GenBank]
Sequencing primer gene specific primer, JS01: 5'-ATTAATCCCCAAATCTTTCACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K020889-022-296-G02C-JS02
CACAAGAAACAATATCTGTCCCAAAATCAGGATCAATAATCTGAGACAGAGCCTTCAACA
CATCTTTTTCTGATGTTTGAGCAACACTCTCACCAACACTACTACTAGCTGTTTTCCAAA
ATGCATAAGTTAAAAAATATCAGAACTTGCTTAGTCTCCTTCGCGTATAACGTTAACAAT
TTTTCAGGCGGAGACAAAGGAACAAACTTTATAACCTTGAGCTGAAGCAGCTTTAGCTAC
AGAGAGATTCTGAGAGACCCGTTTGAGGATTCTTGTCCTGGAGATTGAGATAATGGAAGC
TTGAGAATGGAGAAACTTGTGAGAAAGAAGCAATCTTGTTGTGGAATTGCTTCTTCTTTG
GGTCTGAATCTCGAAAGAAGGATGCCGCAACGACTGTGGATGAAGAAGCGGCATTGCTAC
AAGAGAGAGACGAAGAGAGTGGCAAAAAAAACTAGCGGTCTGTGATAAAATGGCTTCAAA
GCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGAC
GTGTCTA

Last Updated on 06.12.2018 16:11