Sequences from confirmation amplicon


Confirmation sequences for line 297A06 and hit At1g50310 are shown.
Line ID 297A06
Locus At1g50310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18636026
Primers used for PCR 10qw - 8474, show primer details
   
Sequence Name 39-K115837-0022-297-A06C-10qw
GenBank Accession LN503475 [GenBank]
Sequencing primer gene specific primer, 10qw: 5'-ATGACCATGTTGAGTTGTTGACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115837-0022-297-A06C-10qw
ACATCTACCAACGATGAGCATTGCGACATTGGTAGCAAATGCGTTGAATAGAGAACCTAT
GAGGAAAGCAACCCCTCCAACAAACATGGAGATTTTGCGTCCGTACTTCCTTGTGACAGC
CGAAGCTACAAAGGAAGACGCGAGTGCCGCGAGGTAGAGTGAGGAAGTGAACAACTGTAG
CAATTGGTTATCGAATTTGCAGTAAGCAGTCTCGCGCCTTGCTTCGTGCATTTGCTTATC
CACCTCCGGGAAAAACTTGCTCAGAAACTCCTCCATAGATGTCACTCCACCTATACAACA
AAAGAAGTCACGTTACATTCCCCGAAGCTAGAGTGGACAGCGAAAGCGAACCAAAATAAC
TCAACCCGAAGAAGAAAAAGGCGAATGTGGAATTTACGAACCTGAGATACCGAGGTCATA
ACCAAAGAGGAGACCTCCCATAGCGGCCACAATACATGTCATGATGACAAAGACGGTGAC
TCCGCCTTCATAGCTGTTCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATATAGATGTCCG
   
Sequence Name 07-K115833-0022-297-A06C-8409
GenBank Accession LN489418 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115833-0022-297-A06C-8409
CATTTCATTGAATATATCCTGAAGAACAGCTATGAAGGCGGAGTCACCGTCTTTGTCATC
ATGACATGTATTGTGGCCGCTATGGGAGGTCTCCTCTTTGGTTATGACCTCGGTATCTCA
GGTTCGTAAATTCCACATTCGCCTTTTTCTTCTTCGGGTTGAGTTATTTTGGTTCGCTTT
CGCTGTCCACTCTAGCTTCGGGGAATGTAACGTGACTTCTTTTGTTGTATAGGTGGAGTG
ACATCTATGGAGGAGTTTCTGAGCAAGTTTTTCCCGGAGGTGGATAAGCAAATGCACGAA
GCAAGGCGCGAGACTGCTTACTGCAAATTCGATAACCAATTGCTACAGTTGTTCACTTCC
TCACTCTACCTCGCGGCACTCGCGTCTTCCTTTGTAGCTTCGGCTGTCACAAGGAAGTAC
GGACGCAAAATCTCCATGTTTGTTGGAGGGGTTGCTTTCCTCATAGGTTCTCTATTCAAC
GCATTTGCTACCAATGTCGCAATGCTCATCGTTGGTAGATTGTTGCTCGGAGTCGGTGTT
GGATTTGCCAATCAGGTGAGTCAACAACTCACANTGGTCA

Last Updated on 06.12.2018 16:11