Sequences from confirmation amplicon


Confirmation sequences for line 297C03 and hit At4g26990 are shown.
Line ID 297C03
Locus At4g26990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13551195
Primers used for PCR FV82 - 8474, show primer details
   
Sequence Name 16-K100894-0022-297-C03C-8409
GenBank Accession LN493360 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K100894-0022-297-C03C-8409
CATTTACAATTGAATGACAGTTGCAGTTCCTAATGGTTTCTACACAAAGTTTCCATAAAC
AAAGTCTCATTGTTTTTGGTTTCATACCTTTATTCTTTGCCTCTCCAACCTAATAAGGGT
TAAAAAGGTCCTAGTTTTGGTTCCTAGTTTTTTCCTATTCTTCGGTTTTGTGTAAGTATG
TTGCGAGTGTTAAGCTCTGTTTTGGCTTATTATATTGGGGTTTTTTTGCATTGGTTTATC
GGAGTTGAATTTGGGTGAAAAATGTTGTAGAATATGAAAACATTATATGATTGATTCTTT
GGAAAAAGTATCGTTGATCACATATTGGTGATCTCAATAAATTGCAGTTCATAAATGTTG
GAAGTTAAATTACGTCTGAAACAGAGCGAGACTAATCACGTAACATATTACACAAGAGAG
ATTAGAGAGATACAAATGAGAGATCATAAACGTTAAGTCGGTTATGTTTCGGTTTGAGCT
AATTAGAAGTTCATATAATCCGC
   
Sequence Name 16-K100902-0022-297-C03C-FV82
GenBank Accession LN493361 [GenBank]
Sequencing primer gene specific primer, FV82: 5'-TTCTAATCGAGGAATATTAGCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K100902-0022-297-C03C-FV82
CTCAACCGAAACATAACCGACTTAACGTTTATGATCTCTCATTTGTATCTCTCTAATCTC
TCTTGTGTAATATGTTACGTGATTAGTCTCGCTCTGTTTCAGACGTAATTTAACTTCCAA
CATTTATGAACTGCAATTTATTGAGATCACCAATATGTGATCAACGATACTTTTTCCAAA
GAATCAATCATATAATGTTTTCATATTCTACAACATTTTTCACCCAAATTCAACTCCGAT
AAACCAATGCAAAAAAACCCCAATATAATAAGCCAAAACAGAGCTTAACACTCGCAACAT
ACTTACACAAAACCGAAGAATAGGAAAAAACTAGGAACCAAAACTAGGACCTTTTTAACC
CTTATNAGGTTGGAGAGGCAAAGAATAAAGGTATGAAACCAAAAACAATGAGACTTTGTT
TATGGAAACTTTGTGTAGAAACCATTAGGAACTGCAACTGTCATTCAATTGTAAATGGCT
TCA

Last Updated on 06.12.2018 16:11