Sequences from confirmation amplicon


Confirmation sequences for line 297E02 and hit At3g02020 are shown.
Line ID 297E02
Locus At3g02020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:341239
Primers used for PCR 19cs - 8474, show primer details
   
Sequence Name 55-K108895-0022-297-E02C-19cs
GenBank Accession LN508838 [GenBank]
Sequencing primer gene specific primer, 19cs: 5'-GCTTCACAAGTGTCTGTCTCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K108895-0022-297-E02C-19cs
CTCCGCCTCATCATCATTCACTATCAGTGAGATGTTTACCTGATAGCATCCAAAAGTCAG
ACGAGTGTGTATTCAAGGGGTAAACCTTTTGTACAGAATCATCTATATTGCCAAAGAAGT
TGAAAGAGGTTTGACCTTAGATGCACCTTGGGAGATCATCTGGACATTAATCCCATTGGT
TCGAAGTACTCGGAAGCCCTGTTTGTTGTTCATCGGTAAGCCACTATGACTATCTATATA
TTAAATCATACAAACCAAACCAGAGAGTTAACTAACCTTCTCTAATATGAATGATGATCT
CTGAACATTTCCGATGAGAGAGATAATTGATCTGTGTCGAAGCAGATTTACAACAGCAAT
CTTCTCAAGTTCCTCCACCACCTGATCAAGCTCCTGTCACATGGCTTCATGTAAATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGGTTATT
   
Sequence Name 39-K108894-0022-297-E02C-8409
GenBank Accession LN503381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K108894-0022-297-E02C-8409
TTTCATTGAATATTTACATGAAGCCATGTGACAGGAGCTTGATCAGGCGGTGGAGGAACT
TGAGAAGATTGCTGTTGTAAATCTGCTTCGACACAGATCAATTATCTCTCTCATCGGAAA
TGTTCAGAGATCATCATTCATATTAGAGAAGGTTAGTTAACTCTCTGGTTTGGTTTGTAT
GATTTAATATATAGATAGTCATAGTGGCTTACCGATGAACAACAAACAGGGCTTCCGAGT
ACTTCGAACCAATGGGATTAATGTCCAGATGATCTCCCAAGGTGCATCTAAGGTCAAACC
TCTTTCAACTTCTTTGGCAATATAGATGATTCTGTACAAAAGGTTTACCCCTTGAATACA
CACTCGTCTGACTTTTGGATGCTATCAGGTAAACATCTCACTGATAGTGAATGATGATGA
GGCGGAGCATTGTGTGAAGGCTCTCCACTCGGCCTTCTTTGAGACAGACACTTGTGAAG

Last Updated on 06.12.2018 16:11