Sequences from confirmation amplicon


Confirmation sequences for line 297G11 and hit F24G24 are shown.
Line ID 297G11
Locus F24G24 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6436170
Primers used for PCR TF03 - 8474, show primer details
   
Sequence Name 25-K025043-022-297-G11C-8409
GenBank Accession LN497280 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K025043-022-297-G11C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCCACTAACACGTTTTCATT
TGGCCAACTAAGAAAATCTTAATCGTGAGAAATAGCACAAGAGATTTACTCATGTCATCG
AATGCCAGTTTTGCAAAATATTGCGGACCTAAGTTATGTTGGACCTGTTTTACATTGTAG
AATCATCGCGACGAAAATCCCATTGAAAAGAATTTAGATAAAGCGAACTTATGTCTAAAG
CAATTGCTTTAGGTCAATTATTTGATAGTAATATTGTCAATTTTCAATGCTTCTTCACTA
GACTTACACAAAGGGCCAACTTATGTCTAAGGTGATTGCTTTAGGTCATTTAAGTTAATC
TCTTATTTTCTTTTCTTTTTTTGGACAAAAAAGTCATTTAAGTTAGTCTAAACTAGTTAT
ATCTTGTATTATAAAATTTAGTTAAGATAATTCTTTTTTAACTCAAACACCTATTTCCTA
TCCTATGTTTTAATATAAGTGATGTTTAA
   
Sequence Name 26-K025043-022-297-G11C-TF03
GenBank Accession LN497756 [GenBank]
Sequencing primer gene specific primer, TF03: 5'-TGTGCCAAAATCTTAAACATCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K025043-022-297-G11C-tf3
TATTAAAACATAGGATAGGAAATAGGTGTTTGAGTTAAAAAAGAATTATCTTAACTAAAT
TTTATAATACAAGATATAACTAGTTTAGACTAACTTAAATGACTTTTTTGTCCAAAAAAA
GAAAAGAAAATAAGAGATTAACTTAAATGACCTAAAGCAATCACCTTAGACATAAGTTGG
CCCTTTGTGTAAGTCTAGTGAAGAAGCATTGAAAATTGACAATATTACTATCAAATAATT
GACCTAAAGCAATTGCTTTAGACATAAGTTCGCTTTATCTAAATTCTTTTCAATGGGATT
TTCGTCGCGATGATTCTACAATGTAAAACAGGTCCAACATAACTTAGGTCCGCAATATTT
TGCAAAACTGGCATTCGATGACATGAGTAAATCTCTTGTGCTATTTCTCACGATTAAGAT
TTTCTTAGTTGGCCAAATGAAAACGTGTTAGTGGCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11