Sequences from confirmation amplicon


Confirmation sequences for line 298B03 and hit At5g25265 are shown.
Line ID 298B03
Locus At5g25265 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8756100
Primers used for PCR 10pq - 8474, show primer details
   
Sequence Name 42-K115259-0022-298-B03C-10pq
GenBank Accession LN504576 [GenBank]
Sequencing primer gene specific primer, 10pq: 5'-TCATGAAAATAGGCATGGATGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K115259-0022-298-B03C-10pq
ACAATATACAAATTTCAAGAATGCCAAAATGTGTGTATAAGATTACCTGAATCATGAAGT
CTTTGTGTAGAATATTGCTAACACCATGCAATGCAGATGAGACAGCATAAGCATACCTAT
ACTTCCACAAAAGATAACAAACAATTGTCATCAATTCTACTCAATCAACAATCAAAACTA
CTTCTAATCAGAAGACTTTTACATTTCAAGAACCCATCCAAAAGCCTTGTCTGCTTCAGG
ATCCTTCTTCATAGCCAAAGAAACATTCATCCAAGTTGGAGCAATCTTCTTCAAAGCATC
CTAAAAAAACATAACATTTACAATCTTTAAAGGGACCTGATCCCAAATTGTTTTCTAATT
ATATGCAACAAGAACAAACCTTTCCAACAATGACAGGAGAGTTTCCTATCGGATCGATAT
TAGTAACCGGTCCTCTCACTTCAGGATAATACTTCCTCAACACCTTCTCATACTTTTTCG
GTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 02-K115258-0022-298-B03C-8409
GenBank Accession LN487159 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115258-0022-298-B03C-8409
GTATGAGAAGGTGTTGAGGAAGTATTATCCTGAAGTGAGAGGACCGGTTACTAATATCGA
TCCGATAGGAAACTCTCCTGTCATTGTTGGAAAGGTTTGTTCTTGTTGCATATAATTAGA
AAACAATTTGGGATCAGGTCCCTTTAAAGATTGTAAATGTTATGTTTTTTTAGGATGCTT
TGAAGAAGATTGCTCCAACTTGGATGAATGTTTCTTTGGCTATGAAGAAGGATCCTGAAG
CAGACAAGGCTTTTGGATGGGTTCTTGAAATGTAAAAGTCTTCTGATTAGAAGTAGTTTT
GATTGTTGATTGAGTAGAATTGATGACAATTGTTTGTTATCTTTTGTGGAAGTATAGGTA
TGCTTATGCTGTCTCATCTGCATTGCATGGTGTTAGCAATATTCTACACAAAGACTTCAT
GATTCAGGTAATCTTATACACACATTTTGGCATTCTTTGAAATTTGTATATTGTTTTTAT
GCGTGTGGCGGTTATGAATGGAGGCAAAAGTGTATCATCCATGCCTANNTTCATG

Last Updated on 06.12.2018 16:11