Sequences from confirmation amplicon


Confirmation sequences for line 298H05 and hit At2g18460 are shown.
Line ID 298H05
Locus At2g18460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8002624
Primers used for PCR BB31 - 8474, show primer details
   
Sequence Name 33-K104161-0022-298-H05C-BB31
GenBank Accession LN500974 [GenBank]
Sequencing primer gene specific primer, BB31: 5'-GATTTATGCTAGTGCCCAACTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104161-0022-298-H05C-BB31
ATGTGAAAGACATAACTTGAGAATACATCGTGTATACAAAACACTATTAAAGCAATTTAT
TTTGTACAAAAAAAAAAAAAAAAAAAAGAAAACCCCCTTTTAGGGTTTTGGGCCTTTCCC
CACCCAAAACCAAACTTAAAAAAACCTCCCCCAACCCCAAAATTCCAAAATTTTCCCAAT
TTTTGGGAAAACCAAGGGGGGGTGTCCTTTACCCCAAAGCCCCCCTTTCAAATTTGACCG
GGGGGACAAAACTTTAATCCCCCCCCCTCCCCCGGGGGGGGGGGGAAAGGGCCCCCCCCC
GGGCCGGGGAAAAGCCCCCAAACCCCAATTTTTTACCCAATTTAAAAAAACCCCCCCCAA
AACCCCCCCCAAAAAAAGGGGTTTGGGGCTTTTTCAAAAAGGAAATTTCCCTTTGGAAAG
GGCCCCCGGCCCGGGAAATCCCCCGGGGACCCCCAGGGGTAAAAGGGGCAAATGGGGAAA
AAAAAAAGAGTTTTTCCCCTTTTTTTTTCCTTTCCAAAAGGGTAATTCCCC
   
Sequence Name 33-K104162-0022-298-H05C-8409
GenBank Accession LN500975 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104162-0022-298-H05C-8409
CGGACTGAGCCATTTACATTGAATATATCCTGTTCGACATACGCACAAAGCCTTTTCTTT
TGGGTTGTATCTCGGGTGGTTTTTTCTACATATGCTAACAACTCTGGACTCAGAGACTAT
TCACAGGTCAGTAGTTGGAACCTTTGCCATGCCACCTGTGTGAAGTCGATGAATCATAGT
TTTGATCCAACGATCAAATCTGAATTGGAGCTCTCTGGCTAACTGACATCAACCTTTGCT
TATACAAGAATGTTGGAAAGTTTTGGAATTTTAGAGTTTGGAGATGTTTGTCTAAGTCTA
GTTTTAGCTTGGACATAGACAAAATACATAAAAGATGTTCTCTTTTTTTTTTTTTTTTTT
TTGGAAAAAAAAAATTGGTTTAAAAGGGTTTTTTAAACCCAAATTTTTCCCCATTTTTTG
GTTTTCCAACCCGGGGGTTTTAAAAAAAAAAACCCTCCCCTAAAATTTGGG

Last Updated on 06.12.2018 16:11