Sequences from confirmation amplicon


Confirmation sequences for line 298H08 and hit At1g31710 are shown.
Line ID 298H08
Locus At1g31710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11355155
Primers used for PCR 76rn - 8474, show primer details
   
Sequence Name 28-K116083-0022-298-H08C-76rn
GenBank Accession LN498991 [GenBank]
Sequencing primer gene specific primer, 76rn: 5'-TGAATGCACTCGAGTTTTAATCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116083-0022-298-H08C-76rn
ATTGATCACAAGACATGATGATTATACAACAAAACCAACTTATTTAAAACCACATCATTA
TTTCTCGAATCGTTACAGTCCATCATCAAGTAGAACAAATTCATCAATGATATGATTAAG
CTGTTTATTAATCGTTTTTAGGAGTGCACTTTCGAGCGGTGGTAACTTTGACGGGTTTGG
TCTTGAGGACAGGGTTTCGCTCGAAAAAGTTGGTCGGTCGGAGCTCAAAGCCACCGGATA
AAGTAGGCATCGTCGGGTAATCTTCCTGGCTAGGAACATGGTGGAAACCGACGGTGTACC
ACATCACTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCA
   
Sequence Name 12-K116082-0022-298-H08C-8409
GenBank Accession LN491732 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116082-0022-298-H08C-8409
GCCATTTCATTGAATATATAGTGATGTGGTACACCGTCGGTTTCCACCATGTTCCTAGCC
AGGAAGATTACCCGACGATGCCTACTTTATCCGGTGGCTTTGAGCTCCGACCGACCAACT
TTTTCGAGCGAAACCCTGTCCTCAAGACCAAACCCGTCAAAGTTACCACCGCTCGAAAGT
GCACTCCTAAAAACGATTAATAAACAGCTTAATCATATCATTGATGAATTTGTTCTACTT
GATGATGGACTGTAACGATTCGAGAAATAATGATGTGGTTTTAAATAAGTTGGTTTTGTT
TGTATAATCATCATGTCTTTGTTGATCAATTAATTTTTCGTTTTCTCTATTTTCGCGTGA
TTAAAACTCAGNTGCATTCAA

Last Updated on 06.12.2018 16:11