Sequences from confirmation amplicon


Confirmation sequences for line 299A01 and hit At2g22930 are shown.
Line ID 299A01
Locus At2g22930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9760307
Primers used for PCR MZ38 - 8474, show primer details
   
Sequence Name 11-K024066-022-299-A01C-8409
GenBank Accession LN490934 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K024066-022-299-A01C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGTAGAAGATAGATAAGGTTGCTAAGGCA
TGAGCATCGTTTTCACGGTACAACACCTTTGATGAAGGATAACCTGGTGGGGGAACACCT
AATACACCACCAGGGGCAAATGTATAAGCTATTGTTGTTGCAGATACTATCATGTAACTC
ACACTCTTGATCATATGCTCTTTGGCAATTTCTGGAATCCAATGAGCAAAATCAAAAAAG
ATCAAGTCCGGTCTCAGAGCACGAACCGCAGCTTCAACCTGATCGCGAGTGAGATCCAAG
GCTTCCGACAGTAAGTTGTCCATCGAGATTGAGATATCCGAGGTTGTCTCAGCACCAGCA
GGGAGGCCATTGACATGAGGGATTGTGAGAGGGTGAAAGACAATACTGTCTGGGAACAGA
TTGTGATGTTCCAACTGTTTTTGGGCTTTCTTAGGTAGCAAGAAAGTGATTTGATGACCT
TTCT
   
Sequence Name 12-K024066-022-299-A01C-MZ38
GenBank Accession LN491393 [GenBank]
Sequencing primer gene specific primer, MZ38: 5'-CAAACTAGCTGAGAAAGGTCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K024066-022-299-A01C-MZ38
AATCACTTTCTTGCTACCTAAGAAAGCCCAAAAACAGTTGGAACATCACAATCTGTTCCC
AGACAGTATTGTCTTTCACCCTCTCACAATCCCTCATGTCAATGGCCTCCCTGCTGGTGC
TGAGACAACCTCGGATATCTCAATCTCGATGGACAACTTACTGTCGGAAGCCTTGGATCT
CACTCGCGATCAGGTTGAAGCTGCGGTTCGTGCTCTGAGACCGGACTTGATCTTTTTTGA
TTTTGCTCATTGGATTCCAGAAATTGCCAAAGAGCATATGATCAAGAGTGTGAGTTACAT
GATAGTATCTGCAACAACAATAGCTTATACATTTGCCCCTGGTGGTGTATTAGGTGTTCC
CCCACCAGGTTATCCTTCATCAAAGGTGTTGTACCGTGAAAACGATGCTCATGCCTTAGC
AACCTTATCTATCTTCTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11