Sequences from confirmation amplicon


Confirmation sequences for line 299B05 and hit At1g63840 are shown.
Line ID 299B05
Locus At1g63840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23690541
Primers used for PCR IS80 - 8474, show primer details
   
Sequence Name 85-K027491-022-299-B05C-8409
GenBank Accession LN514484 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K027491-022-299-B05C-8409
CTTTACAATTGAATATAGATTAAGCCATGTAGATTCTCTCTCTCTGTCTCTTGTCTTATC
ATTTATCTCTCTAACGGCGAGGCAATGGGTTTTCCGGTGGGATATTCTGAGCTACTTCTC
CCAAAAATCTTTTTCTACTTGTTATCTTTCTTAGGTTTGATACGTAAACTCATCTCCACA
ATGTTCAAGATCATTGGTCTACCAGATTTCTTAGAACCTGAACCGGTTTCAACCTCATGG
CCCGACCCACCACCCACGTTGACCAAACCTGACTCAGCAGCAATACTAGCCGGAGAGATG
TTACCGGTTGTTCGATTCTCGGATATAAACCGACCGGAATCCGAGTGTTGCGCAGTGTGT
CTCTACGATTTCGAGAACGACGACGAGATCCGACGGCTGACGAATTGTCGACATATATTT
CATCGGGGATGTTTGGACCGTTGGATGATGGGTTATAATCAGATGACGTGTCCACTTTGT
CGTACACAGTTTATACCTGATCATCTTCAACTGGAGTTTAATCAAAGGCTCTGGTCTCAA
TCTTCT
   
Sequence Name 86-K027491-022-299-B05C-IS80
GenBank Accession LN514597 [GenBank]
Sequencing primer gene specific primer, IS80: 5'-TGAGAAGAAACAGCAGAAGATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K027491-022-299-B05C-IS80
ATCAGGTATAAACTGTGTACGACAAAGTGGACACGTCATCTGATTATAACCCATCATCCA
ACGGTCCAAACATCCCCGATGAAATATATGTCGACAATTCGTCAGCCGTCGGATCTCGTC
GTCGTTCTCGAAATCGTAGAGACACACTGCGCAACACTCGGATTCCGGTCGGTTTATATC
CGAGAATCGAACAACCGGTAACATCTCTCCGGCTAGTATTGCTGCTGAGTCAGGTTTGGT
CAACGTGGGTGGTGGGTCGGGCCATGAGGTTGAAACCGGTTCAGGTTCTAAGAAATCTGG
TAGACCAATGATCTTGAACATTGTGGAGATGAGTTTACGTATCAAACCTAAGAAAGATAA
CAAGTAGAAAAAGATTTTTGGGAGAAGTAGCTCAGAATATCCCACCGGAAAACCCATTGC
CTCGCCGTTAGAGAGATAAATGATAAGACAAGAGACAGAGAGAGAGAATCTACATGGCTT
AATCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTNTAT
NTCGACGTGTCTA

Last Updated on 06.12.2018 16:11