Sequences from confirmation amplicon


Confirmation sequences for line 300F09 and hit At1g62880 are shown.
Line ID 300F09
Locus At1g62880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23292550
Primers used for PCR 14ta - 8474, show primer details
   
Sequence Name 33-K116871w-0022-300-F09C-14ta
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 14ta: 5'-TACTTTGGTTCCATCGACTCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116871w-0022-300-F09C-14ta
AAATTTAGTGTTTTGTGTTCTTCCAATCATAAATCGTTTATAAGAAATGTTTCTAAGTTT
TGGGAATGTGATAAAGTTAGTAACTTTAGGGTTTCTTTATATAATAACTAGTTAATCTGT
CAACTAGAAACAATTTTGTTTCTTAAATTCTTCAATTGCTTCTTGATATATGTGTATTGA
ATCTGTAGATGTTATCTTCTCAGTTTATATCAATCCAGCAAGTATCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 13-K116870w-0022-300-F09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K116870w-0022-300-F09C-8409
ATCCTGATACTTGCTGGATTGATATAAACTGAGAAGATAACATCTACAGATTCAATACAC
ATATATCAAGAAGCAATTGAAGAATTTAAGAAACAAAATTGTTTCTAGTTGACAGATTAA
CTAGTTATTATATAAAGAAACCCTAAAGTTACTAACTTTATCACATTCCCAAAACTTAGA
AACATTTCTTATAAACGATTTATGATTGAAAGAACACAAAACACTAAATTTAGTTCAATA
CAGTGATCGAATAAGCCTAAGCAGAGTTTATTCAAATACAACAAGCAAGAAGAGTCGATG
AAACCAAAGT

Last Updated on 06.12.2018 16:11