Sequences from confirmation amplicon


Confirmation sequences for line 300G05 and hit At3g04800 are shown.
Line ID 300G05
Locus At3g04800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1315040
Primers used for PCR PF74 - 8474, show primer details
   
Sequence Name 29-K103231-0022-300-G05C-8409
GenBank Accession LN499243 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103231-0022-300-G05C-8409
TCGGACTGAGCCTTTACAATTGAATATATCCTGGATCGATGAACCATAGCACCGGGCATC
AACAACAGCAGAAGTACCGTCAGTACAATCCTTACCAACAAGTCAATCTTCCGTACCGTA
AGCTCTACGAACTCCCAACTTCTCCTGAGTTTCTCTTCGAAGAAGAAGCTACGAAAAAAC
GCTTAACATGGGGAGAGAACCTCACCTTCTTCACCGGTTGGGGTTATTGCACTGGATCTG
TTCTCGGAGCCTTCAAGGGTACAATTGCGGGGATGCGAGCCGCCGAACGAGGCGAATCCC
TAAAGATCCGAACTAATCGGATTTTGAACTCCGGTGGACTCGTTGCAAGACGCGGCGGGA
ATTGCTTAGGATCCGTAGGGTTGATGTTTGCTGCTATGGAGAGCGGTGTTACGTATATGA
GAGACGGAGACGACGGTTCGTTGACAACTGTGATCGCTGGTTTAGCCACTGGTGTGCTTT
ACAGAGCTGCTTCTGGACCTAGATCCGCTGTGGTTGCCGGAGCTGTCGGAGGAGTTGCGG
CTTTGGCTGCAGTTGCTGGAAGACGCATCGTCAAGCGATTCGTTCCAATCTAATGAAAGT
TTCTCAATCTCTCANGGTTAGGGTTTAAGAACTAGTTTAAAGGAGGGGCTTTT
   
Sequence Name 77-K103232-0022-300-G05C-PF74
GenBank Accession LN513533 [GenBank]
Sequencing primer gene specific primer, PF74: 5'-GATTCACTATCTAAAAGCCCCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K103232-0022-300-G05C-PF74
ACCTACCTTGAGAGATTGAGAACTTTCATTAGATTGGAACGAATCGCTTGACGATGCGTC
TTCCAGCAACTGCAGCCAAAGCCGCAACTCCTCCGACAGCTCCGGCAACCACAGCGGATC
TAGGTCCAGAAGCAGCTCTGTAAAGCACACCAGTGGCTAAACCAGCGATCACAGTTGTCA
ACGAACCGTCGTCTCCGTCTCTCATATACGTAACACCGCTCTCCATAGCAGCAAACATCA
ACCCTACGGATCCTAAGCAATTCCCGCCGCGTCTTGCAACGAGTCCACCGGAGTTCAAAA
TCCGATTAGTTCGGATCTTTAGGGATTCGCCTCGTTCGGCGGCTCGCATCCCCGCAATTG
TACCCTTGAAGGCTCCGAGAACAGATCCAGTGCAATAACCCCAACCGGTGAAGAAGGTGA
GGTTCTCTCCCCATGTTAAGCGTTTTTTCGTAGCTTCTTCTTCGAAGAGAAACTCAGGAG
AAGTTGGGAGTTCGTAGAGCTTACGGTACGGAAGATTGACTTGTTGGTAAGGATTGTACT
GACGGTACTTCTGCTGTTGTTGATGCCCGGTGCTATGGTTCATCGATCCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTC
TACTT

Last Updated on 06.12.2018 16:11