Sequences from confirmation amplicon


Confirmation sequences for line 301H05 and hit At2g44430 are shown.
Line ID 301H05
Locus At2g44430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18335128
Primers used for PCR 16uj - 8474, show primer details
   
Sequence Name 29-K117387-0022-301-H05C-16uj
GenBank Accession LN499459 [GenBank]
Sequencing primer gene specific primer, 16uj: 5'-CTTCTTTCGTATCATCTTTTTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117387-0022-301-H05C-16uj
ACGGAACGAACGCCTCTTCTTACAAACGACAAGAGGACCTGAAGATTTCTGCCTAGAGAG
AGACGAGTCTGATGTTTCAGCATCCGCTTTGCCACTACGCATACCGGATGCTTCCTGTTT
TATTAACCTGGGGCCAGCTTTTCCGGTCTCTTTTCTCATTTCTTGAGAAACAACAGCTCG
TAGTTCGTGAGCTGCCATCGATTCTGAGGAAGATAAAGGAAAGAAGACAATTGCGTTTGT
GAAGAGAAGCTGTAGATCTCTGTAAAAAATGAGGCTTGAAGAATCATAAGATCCTTGCTT
CAACTTTCTCTGTATTGTCTCAATGTCCAAATGTTGCTTAACCATGCTCTTGTAATCCTT
AGCCTCCTGCGTTTTAATGGATTTATAAACGTAAGTTACAAATATAGGCCCATTAGTACC
AACTAAAAGCCCAAAATAAATAAAAAGTTAGCGGGAAGACACGATAAATCCAGACCATCA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCG
   
Sequence Name 02-K117386-0022-301-H05C-8409
GenBank Accession LN487211 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117386-0022-301-H05C-8409
CATTTCATTGATATGATGGTCTGGATTTATCGTGTCTTCCCGCTAACTTTTTATTTATTT
TGGGCTTTTAGTTGGTACTAATGGGCCTATATTTGTAACTTACGTTTATAAATCCATTAA
AACGCAGGAGGCTAAGGATTACAAGAGCATGGTTAAGCAACATTTGGACATTGAGACAAT
ACAGAGAAAGTTGAAGCAAGGATCTTATGATTCTTCAAGCCTCATTTTTTACAGAGATCT
ACAGCTTCTCTTCACAAACGCAATTGTCTTCTTTCCTTTATCTTCCTCAGAATCGATGGC
AGCTCACGAACTACGAGCTGTTGTTTCTCAAGAAATGAGAAAAGAGACCGGAAAAGCTGG
CCCCAGGTTAATAAAACAGGAAGCATCCGGTATGCGTAGTGGCAAAGCGGATGCTGAAAC
ATCAGACTCGTCTCTCTCTAGGCAGAAATCTTCAGGTCCTCTTGTCGTTTGTAAGAAGAG
GCGTTCTGTTTCCGCTAAGGCTTCTCCTTCGTCATCAAGCTTTAGCCAAAAA

Last Updated on 06.12.2018 16:11