Sequences from confirmation amplicon


Confirmation sequences for line 302E01 and hit At4g02850 are shown.
Line ID 302E01
Locus At4g02850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1267880
Primers used for PCR 03bn - 8474, show primer details
   
Sequence Name 47-K107839-0022-302-E01C-03bn
GenBank Accession LN506215 [GenBank]
Sequencing primer gene specific primer, 03bn: 5'-GAAAATTCGACAGTGTCAGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K107839-0022-302-E01C-03bn
TATGAGCTGATGCTAGTGTTGCATGACCACATATATCCATCTACAAAATCAAATAAATCG
AACATTGTACAAAATGGAACGGAGGGAGTAATAAAATACAAAAATGTTTGCAAGATGGAC
CTCGGCTACAGCCGTGAACCACCTCAGAAGGAAACGTGGTGGTTCGGAGCCGGGGATGGG
AATAAGAAAACACGTCAATGGAAGGTCAAACTCAGAGGCCATAGACTGAAGCCACACGTC
GTCTCTCTCATGATCCTCTTCAAGAAAACACACTGCAGCTTGGTTCCCCTTGAAGGCTGA
CTCAGTGAATGCATCAACCTGTGTTTAGCAATACTCGTTCATAAAGAAGAAGAAGATCAC
ATTATCAGAGCAAAAACAAAGAAAAGAATATACAATTACCACGAAGTACTTGACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTA
   
Sequence Name 79-K107840-0022-302-E01C-8409
GenBank Accession LN513833 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K107840-0022-302-E01C-8409
ATTTACATTGAATATATCCTGTCAAGTACTTCGTGGTAATTGTATATTCTTTTCTTTGTT
TTTGCTCTGATAATGTGATCTTCTTCTTCTTTATGAACGAGTATTGCTAAACACAGGTTG
ATGCATTCACTGAGTCAGCCTTCAAGGGGAACCAAGCTGCAGTGTGTTTTCTTGAAGAGG
ATCATGAGAGAGACGACGTGTGGCTTCAGTCTATGGCCTCTGAGTTTGACCTTCCATTGA
CGTGTTTTCTTATTCCCATCCCCGGCTCCGAACCACCACGTTTCCTTCTGAGGTGGTTCA
CGGCTGTAGCCGAGGTCCATCTTGCAAACATTTTTGTATTTTATTACTCCCTCCGTTCCA
TTTTGTACAATGTTCGATTTATTTGATTTTGTAGATGGATATATGTGGTCATGCAACACT
AGCATCAGCTCATAGTATCTTCTCAAACAGTTTGGTTGGTTCATCTGACACTGTCGAATT
TT

Last Updated on 06.12.2018 16:11