Sequences from confirmation amplicon


Confirmation sequences for line 302E02 and hit At3g47730 are shown.
Line ID 302E02
Locus At3g47730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17598221
Primers used for PCR 48qa - 8474, show primer details
   
Sequence Name 57-K115305-0022-302-E02C-48qa
GenBank Accession LN509505 [GenBank]
Sequencing primer gene specific primer, 48qa: 5'-GACAACCAATAAGCAGTGTCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K115305-0022-302-E02C-48qa
CATCTACTATAAAGACTTTGTTCCAGCAGCCTATATAGAAACAACGTTATACCTGGCGAA
GTTTAAGCTCTTTCTCAGTAATCAAAGAACTAATCTGAAGTACAAAACCAAACATAGCAA
CCGCAAGGAAAAAAGTCGGTCCTATAGTGTCCAACGCAACAATAGCTTCAATAGTTGGGT
GAGGAAACTCCTTAAACCCAACAACCCAATTAAAGTTCGGATCTGCAAAAGCGTAACAAC
AACTTCTCAACGTCAAGAACTCAACACTCATGACCACACACTCAAAAGATTCAACAAGAT
AAAATGCATACCTCCTATCAACGACCTCGCGATTTCACGCTCAGCCGCAATTTGAAGAGG
TATCTGAAACTTAAAAGTAGGATCTTCAAACCTTCCTCTATTCATCTCTGGAGTTGAGTT
AGTTTGAATACCATAACTTATCACTGTAGCATTTCTTTCAATAAAGTGCAAAGCTCCTGG
AACTAACAAAGGATTCGCCATAAGCCACGTATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 22-K115304-0022-302-E02C-8409
GenBank Accession LN496242 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115304-0022-302-E02C-8409
TTGAATACGTGGCTTATGGCGAATCCTTTGTTAGTTCCAGGAGCTTTGCACTTTATTGAA
AGAAATGCTACAGTGATAAGTTATGGTATTCAAACTAACTCAACTCCAGAGATGAATAGA
GGAAGGTTTGAAGATCCTACTTTTAAGTTTCAGATACCTCTTCAAATTGCGGCTGAGCGT
GAAATCGCGAGGTCGTTGATAGGAGGTATGCATTTTATCTTGTTGAATCTTTTGAGTGTG
TGGTCATGAGTGTTGAGTTCTTGACGTTGAGAAGTTGTTGTTACGCTTTTGCAGATCCGA
ACTTTAATTGGGTTGTTGGGTTTAAGGAGTTTCCTCACCCAACTATTGAAGCTATTGTTG
CGTTGGACACTATAGGACCGACTTTTTTCCTTGCGGTTGCTATGTTTGGTTTTGTACTTC
AGATTAGTTCTTTGATTACTGAGAAAGAGCTTAAACTTCGCCAGGTATAACGTTGTTTCT
ATATAGGCTGCTGTGAACAAAGTCTTTATAGTAGATTGATTGTGTTGTGCAACAGGCAAT
GACGATGATGGGTGTTTTTGACACTGCTTATTGGTTGT

Last Updated on 06.12.2018 16:11