Sequences from confirmation amplicon


Confirmation sequences for line 302H05 and hit At5g12370 are shown.
Line ID 302H05
Locus At5g12370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4007307
Primers used for PCR 08um - 8474, show primer details
   
Sequence Name 31-K117403-0022-302-H05C-08um
GenBank Accession LN500323 [GenBank]
Sequencing primer gene specific primer, 08um: 5'-AAAAGCCTGTTCTCCAATTCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117403-0022-302-H05C-08um
AACATACAATAATCCCTTTTCAGTAAATATACAGAAAACACAGTATAAACGAATTAGTTT
ACCATTACAGTAATCCTGAAGATTAGCAACTGCAACTTCCAGTCCTCGGCCAGGGGTAGC
ATTTCCTGCAGCTGCACTCGCACCTTGCCTTCCAATGTCTTCCTCAGCAAAGGATCCTGA
TATCGACAAGGAGAGCATGGAAATCAATTGCAGCCTAATTTAACTGTAAATTTTCATTGT
TTCTTTTTGGTTTTAGTAAAGTTTCGTTGTTGGAGAGTTAAATTCTTAGTTGTTAACAAA
TGATAGATAAAAAGTTGTTCTAACACAAAAATGTAATTACCAATTTTTTAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 07-K117402-0022-302-H05C-8409
GenBank Accession LN489453 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117402-0022-302-H05C-8409
TTAAAAATCGGTAATTACATTTTTTGTGTTAGAACAACTTTTTATCTATCATTTGTTAAC
AACTAAGAATTTAACTCTCCAACAACGAAACTTTACTAAAACCAAAAAGAAACAATGAAA
ATTTACAGTTAAATTAGGCTGCAATTGATTTCCATGCTCTCCTTGTCGATATCAGGATCC
TTTGCTGAGGAAGACATTGGAAGGCAAGGTGCGAGTGCAGCTGCAGGAAATGCTACCCCT
GGCCGAGGACTGGAAGTTGCAGTTGCTAATCTTCAGGATTACTGTAATGGTAAACTAATT
CGTTTATACTGTGTTTTCTGTATATTTACTGAAGAGGATTATTGTATGTTCAACTTTTTC
TTTGTGACATTTGAGTATGATGCTGTAGAATTG

Last Updated on 06.12.2018 16:11