Sequences from confirmation amplicon


Confirmation sequences for line 303C11 and hit At4g26210 are shown.
Line ID 303C11
Locus At4g26210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13282890
Primers used for PCR 19wx - 8474, show primer details
   
Sequence Name 53-K118966-0022-303-C11C-19wx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 19wx: 5'-GCCTAGAGATTCTGTTGTTGATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118966-0022-303-C11C-19wx
CAGCCAGGTAAGAGACATTAGTTTTTATTTTGTTTCGAAGTTTCATCTCTTTTCTTTCCA
CTTTATCCCCTTCAAGGAATTTGTGCTAGATCTACGCTTTTAGGTCGCTGGCATTTGATT
GATTTTGGACCAACAAAAGCTAATTTAAATGTTCATGAGGTCTTGAGATTTGATGATTAG
GCTTTTATTTTGTCGAGACTGATGTTAGGTCGAACTATGTTGATTTAGGTTTGGGATTCG
ATTTGATTTTATGGAATTGGATTCCTTAGATAGATCCTTTATATGCATCCGTTAGGTCCT
CTTTTAATTTGTAATTCTAAACTGTGTCTTAGGGAAGAGTTTTAAGAGATGGCATCAAAG
TTGCTACAATTGAAATCCAAGGCATGTGAAGCATCAAAGTTTGTTTCCAAGCATGGAACA
ACTTACTACAAACAGTTGCTGGATAAGAACAAGATGTATATCCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 29-K118965-0022-303-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118965-0022-303-C11C-8409
ATGCTTGGAAACAAACTTTGATGCTTCACATGCCTTGGATTTCAATTGTAGCAACTTTGA
TGCCATCTCTTAAAACTCTTCCCTAAGACACAGTTTAGAATTACAAATTAAAAGAGGACC
TAACGGATGCATATAAAGGATCTATCTAAGGAATCCAATTCCATAAAATCAAATCGAATC
CCAAACCTAAATCAACATAGTTCGACCTAACATCAGTCTCGACAAAATAAAAGCCTAATC
ATCAAATCTCAAGACCTCATGAACATTTAAATTAGCTTTTGTTGGTCCAAAATCAATCAA
ATGCCAGCGACCTAAAAGCGTAGATCTAGCACAAATTCCTTGAAGGGGATTGAGTGGAAA
GAAAAGAGATGAAACTTCGAAACAAAATAAAAACTAATGTCTCTTACCTGGATGATGTAA
CCGGAGACGGAGATTGTACTACCGGAGAAGGAGAGCGGGAATCGGATCATCTGCAGAATC
TGGAGG
Primers used for PCR 65xp - 8474, show primer details
   
Sequence Name 28-K119356-0022-303-C11C-65xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 65xp: 5'-GCCTTGATAAAAGGCGTGTAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K119356-0022-303-C11C-65xp
TCTTCAAAACCAATTGATTGAGCATACAGTTGACTTATGAATCAAGTTTATGTTCACTAT
CAAAACACTCATAAAAAAAAAGAACAACTACAAAACTAACGATCCAAAATGATCACAAAA
ATGTTACAAAGAGAAGACTTATAGTGAGGACCGGTCGAGAGAGAGAGAGGAGAATACCAG
ATGACTGAAGTCTTAGTTTCCGATCATCCACTACAATCTGGTGTGATGGCTTCTACACCT
ACAAGGCTTGCTTTTATGCGAAATTGCGATAGACAAATGACAGAGACAACGTTGAAATAC
TCTGAACCTAATCGTATTTTCAGACAATTGTCAAAAATATAGGATTTTCTTCCTTTTTTG
TGATAGAAGAAGAATGATACATATTACCAAACGGTTTATAATATAATGCCGTCTTACTCT
TCTCTGGTAAGGTTGGTATGTGCTGAGGTTTCACGAATGATACATCTTACCAAACGGTTT
ATAGACTCTAAACCCAAGAAAAATGGTTATTCACATAAATGAAAATGACAAAGAAAAAAC
CAAATGGCCAACATTAGTCGACTGATATTATTAGAGCTAGCACGAGTGATGATGCCTCAC
CTTCAAAACTTTTGTGTATAAATTATCCAGAGAAACAGAAATTAACTTTGCTTCTAATGT
CAGCAGTGAGATAGATAATAAGCCGCTAATTATGATATAAAAAGGCTTTTCGGTATTCAA
TGGATCTGAAACGCATGTTTGTTTATGAGGGGGTTGCTTTCTTTCTTCA
   
Sequence Name 08-K119355-0022-303-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119355-0022-303-C11C-8409
TCGGATGAGCCATTTCATTGAATTATCCTGACTTGTTTGTTGCTATGACTGACTTGTTTT
TTTCAAATGGATCGTAGCATTCCTGGGCGTTCTGAGTCATTTTGGAAGGAAGTCAATCAC
GTGAAGGGTTTATGGAAGAATCGGGCGGATTTGAAGGTTGAAGATGCTGGAATAGCTGCA
CTTTTTGGTCTGGAATGCTTTGCGTGGTACTGTGCAGGAGAGATTGTAGGAAGGGGCTTC
ACTTTCACCGGCTACTACCCTTGAAGAAAGAAAGCAACCCCCTCATAAACAAACATGCGT
TTCAGATCCATTGAATACCGAAAAGCCTTTTTATATCATAATTAGCGGCTTATTATCTAT
CTCACTGCTGACATTAGAAGCAAAGTTAATTTCTGTTTCTCTGGATAATTTATACACAAA
AGTTTTGAAGGTGAGGCATCATCACTCGTGCTAGCTCTAATAATATCAGTCGACTAATGT
TGGCCATTTGGTTTTTTCTTTGTCATTTTCATTTATGTGAATAACCATTTTTCTTGGGTT
TAGAGTCTATAAACCGTTTGGTAAGATGTATCATTCGTGAAACCTCAGCACATACCAACC
TTACCAGAGAAGAGTAAGACGGCATTATATTATAAACCGTTTGGTAATATGTATCATTCT
TCTTCTATCACAAAAAAGGAAGAAAATCCTATATTTTTGACAATTGTCTGAAAATACGAT
TAGGTTCAGAGTATTTCAACGTTGTCTCTGTCATTTGTCTATCGCAATTTCGCATAAAAG
CAAGCCTTGTAGGTGTAGAAGCCATCACACCAGATTGTAGTGGATGATCGGAAACTAAGA
CTTCAGTCATCTGGTATTCTCCTCTCTCTCTCTCGACCGGTCCTCACTATAAATCTTC

Last Updated on 06.12.2018 16:11