Sequences from confirmation amplicon


Confirmation sequences for line 304D03 and hit At5g13930 are shown.
Line ID 304D03
Locus At5g13930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4488603
Primers used for PCR PH39 - 8474, show primer details
   
Sequence Name 22-K017545-022-304-D03C-PH39
GenBank Accession LN495902 [GenBank]
Sequencing primer gene specific primer, PH39: 5'-ATGCCAAAGGAAATGAAAGTAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K017545-022-304-D03C-PH39
TAAGACGTACACATGCGCTTGAACTTCTCCTTGAGGTCGGTCATGTGTTCACTGTTGGTG
ATGCGGAAGTAGTAGTCAGGATACTCCGCCTGAAGCACATGGTTCTCAGGGTTAGCAGTG
CCAATAGCCAAGATGCCTGCAGGTCCATCAGCTCTCTGAGCCTGTCTGATCTCATCCAAA
GAAGAAGCACCAGCCATCACCATTATAGTATACACCAACTTGGGTTTATTAGAGTTTGAT
GTTGCTGTTGTGTACTAAACAAGTTAGGTGTATTTGGAAATATACCGGAGAGTGAGAGCT
TATATAACAAACTGACACGGGAAAAAGAAGATGAGAGTGTGGTAGGTTAGATGGACGGGC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCA
   
Sequence Name 26-K017545-022-304-D03C-8409
GenBank Accession LN497703 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K017545-022-304-D03C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCCCGTCCATCT
AACCTACCACACTCTCATCTTCTTTTTCCCGTGTCAGTTTGTTATATAAGCTCTCACTCT
CCGGTATATTTCCAAATACACCTAACTTGTTTAGTACACAACAGCAACATCAAACTCTAA
TAAACCCAAGTTGGTGTATACTATAATGGTGATGGCTGGTGCTTCTTCTTTGGATGAGAT
CAGACAGGCTCAGAGAGCTGATGGACCTGCAGGCATCTTGGCTATTGGCACTGCTAACCC
TGAGAACCATGTGCTTCAGGCGGAGTATCCTGACTACTACTTCCGCATCACCAACAGTGA
ACACATGACCGACCTCAAGGAGAAGTTCAAGCGCATGTGTACGTCTTATTAACTTCTACT
TTCATTT

Last Updated on 06.12.2018 16:11