Sequences from confirmation amplicon


Confirmation sequences for line 305A04 and hit At2g24180 are shown.
Line ID 305A04
Locus At2g24180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10282779
Primers used for PCR IF63 - 8474, show primer details
   
Sequence Name 12-K101423-0022-305-A04C-8409
GenBank Accession LN491511 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K101423-0022-305-A04C-8409
GCCATTTACATTGAATATGTGTATCGGTGATTCTTGAAGAACCAGTCTTGTTTCTTGACT
CTGTAACTTGAGCAAGACGTCAATGAGATCTTCACGGTCTTCAGTTTTCTTCTTCTCCCG
ATGGTCAACAATAGCTTTCTGGTAAAACGTATCAAGATCTTGAAAACTCTTCCTCCGGCT
AGAATCAAGACCACTGATCCGATCCAAGATCCAGCCAAACCCGGGGAATATATCCGCCGC
CGCGAATTTTCCGATCACTCTGTTCGCCTCCGTGAACAGCTCCAGAAACGTCTCGTTATC
CATTCCACTTCCTTGAAAACTCAATCCAAACGCCATTCGACACGTAAAGCTCGCGACGAA
CTTAGCCAAATTTGCGGTAAGGTCAACGGAAGATCCAGATGACGCCGAGTCAGAAAACGA
GTTAACGAGTCGACTCAGCTCCTCCTCTCTNATGTTCCGGAACGAGTTGGCTCGTTTAAC
GGAGAAAAGTTCAAGAACACAGAGTTTCCGCACATCTCTGTAGTAATCACCCCATTTAGT
AAACCCTAGACCAAGTCCATCANAGAAAAAGCTTTTGGTGAT
   
Sequence Name 52-K101426-0022-305-A04C-IF63
GenBank Accession LN507761 [GenBank]
Sequencing primer gene specific primer, IF63: 5'-CCAAAGCTATCTATCACCAAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101426-0022-305-A04C-IF63
TGGGGTGATTCTACAGAGATGTGCGGAAACTCTGTGTCCTTGAACTTTTCTCCGTTAAAC
GAGCCAACTCGTTCCGGAACATAAGAGAGGAGGAGCTGAGTCGACTCGTTAACTCGTTTT
CTGACTCGGCGTCATCTGGATCTTCCGTTGACCTTACCGCANATTTGGCTAAGTTCGTCG
CGAGCTTTACGTGTCGAATGGCGTTTGGATTGAGTTTTCAAGGAAGTGGAATGGATAACG
AGACGTTTCTGGAGCTGTTCACGGAGGCGAACAGAGTGATCGGAAAATTCGCGGCGGCGG
ATATATTCCCCGGGTTTGGCTGGATCTTGGATCGGATCAGTGGTCTTGATTCTNGCCGGA
GGAAGAGTTTTCAAGATCTTGATACGTTTTACCAGAAAGCTATGGTTGACCATCGGGAGA
AGAAGAAAACTGAAGACCGTGAAGATCTCATTGACGTCTTGCTCAAGTTACAGAGTCAAG
AAACCAAACTTGGTTCTTCAAGAATCACCGATACACATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG

Last Updated on 06.12.2018 16:11