Sequences from confirmation amplicon


Confirmation sequences for line 305E10 and hit At1g78040 are shown.
Line ID 305E10
Locus At1g78040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29346629
Primers used for PCR BV12 - 8474, show primer details
   
Sequence Name 70-K106189-0022-305-E10C-BV12
GenBank Accession LN512394 [GenBank]
Sequencing primer gene specific primer, BV12: 5'-AATACAATGGTGGTTCAAGGTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K106189-0022-305-E10C-BV12
ATTCGGCTCGAGACTCCTGAATCTCCTACTTCATCCCCGGTATATCTCTATCTCTCTAAA
TCCTTAGTTTCTGCATTCTAAGATCTAAGAAGTTTCCAATGTTAACGTTCTCTTTAGTGT
TTGATTCTCGATTCTAATGAAGTAGTTTGTTAGATCTGAGTTCCGAATCTCGACTTTGAA
TGGATTAGTTAGTTAGATCTGAGAATTTTACTTATAGTTTGATTCTCTATGCTCTCGTAG
TGTTAACTGCTTTTGCTCTAGATCTGGCTTTAGGATTGATAAATACAGCTTTGTTCATTG
ATTGTGGTGAGAATTGTTTTGTCCTTCAAGTTCATAGATCTTGATTGTTGTGGTGGTAAA
TGATTGTTCAGGTGCAACGGTGAAGCTATCATGCAAAGACAGGAAGACAATGGAAGAGGT
TTACACAGACAAAGCTGTATCAGACAAAGAAGGAAAGTATAAGTTCATTGTCCACGACGA
TCACAGAGACCAGATGTGCGATGTTTTGCTTGTGAAAAGTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 62-K106190-0022-305-E10C-8409
GenBank Accession LN510764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K106190-0022-305-E10C-8409
ACTTTTACAGCAAAACATCGCACATCTGGTCTCTGTGATCGTCGTGGACAATGAACTTAT
ACTTTCCTTCTTTGTCTGATACAGCTTTGTCTGTGTAAACCTCTTCCATTGTCTTCCTGT
CTTTGCATGATAGCTTCACCGTTGCACCTGAACAATCATTTACCACCACAACAATCAAGA
TCTATGAACTTGAAGGACAAAACAATTCTCACCACAATCAATGAACAAAGCTGTATTTAT
CAATCCTAAAGCCAGATCTAGAGCAAAAGCAGTTAACACTACGAGAGCATAGAGAATCAA
ACTATAAGTAAAATTCTCAGATCTAACTAACTAATCCATTCAAAGTCGAGATTCGGAACT
CAGATCTAACAAACTACTTCATTAGAATCGAGAATCAAACACTAAAGAGAACGTTAACAT
TGGAAACTTCTTAGATCTTAGAATGCAGAAACTAAGGATTTAGAGAGATAGAGATATACC
GGGGATGAAGTAGGAGGATTCAGGAGTCTCGAAGCCGAATTTGCAAATGTCACAGTAGGT
GCTACCTTGA

Last Updated on 06.12.2018 16:11