Sequences from confirmation amplicon


Confirmation sequences for line 305H10 and hit At3g58640 are shown.
Line ID 305H10
Locus At3g58640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21687583
Primers used for PCR 05ie - 8474, show primer details
   
Sequence Name 34-K112875-0022-305-H10C-8409
GenBank Accession LN501479 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K112875-0022-305-H10C-8409
TTGAATATATAGCAATTGAAGTCATTGCGGATAAGTTCAGGAGCCATCCACTCTGGAGTT
CCTGCAGAGACTGTGTCTCTCATTGTTGTCCCTGTCATTATTCTCGACAGCCCAAAATCA
CAGATCTTGACTGTCCATTTGTTGCTCAAAAGACAGTTTGCACTCTTTATATCACGGTGT
ACTATCCCCATCCGGTGTATACACATCAATCCCCTAGCAACATTTTACAAGTTTAAACGC
TGTTTGTTAATTAAAGATGATTACGAAACTTTCTCATCGAGACACAATTCCAGGTCCTAT
TTGGAAGTGTTATTGCCTTCTCCTCAAACCAAAATTTTCCATATTAATACTACATACTAT
GTTGGAAAGAAGGATACACGGTTTTGNTATGGAAG
   
Sequence Name 50-K112876-0022-305-H10C-05ie
GenBank Accession LN507224 [GenBank]
Sequencing primer gene specific primer, 05ie: 5'-CTTCCATACAAAACCGTGTATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K112876-0022-305-H10C-05ie
TATGGAAATTTTGGTTTGAGGAGAAGCAATAACACTTCCAAATAGGACCTGGAATTGTGT
CTCGATGAGAAAGTTTCGTAATCATCTTTAATTAACAAACAGCGTTTAAACTTGTAAAAT
GTTGCTAGGGGATTGATGTGTATACACCGGATGGGGATAGTACACCGTGATATAAAGAGT
GCAAACTGTCTTTTGAGCAACAAATGGACAGTCAAGATCTGTGATTTTGGGCTGTCGAGA
ATAATGACAGGGACAACAATGAGAGACACAGTCTCTGCAGGAACTCCAGAGTGGATGGCT
CCTGAACTTATCCGCAATGACTTCAATTGCTTATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATA

Last Updated on 06.12.2018 16:11