Sequences from confirmation amplicon


Confirmation sequences for line 306B12 and hit At4g25090 are shown.
Line ID 306B12
Locus At4g25090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12882845
Primers used for PCR 06ak - 8474, show primer details
   
Sequence Name 23-K106739-0022-306-B12C-06ak
GenBank Accession LN496572 [GenBank]
Sequencing primer gene specific primer, 06ak: 5'-ATGGTATCTGGAGAGAGTAAGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106739-0022-306-B12C-06ak
CGGCCGGTCAGGCCTTGAAGGGCTCAAGATCATCAGTAAGACCGACGGTACCGCTGCCTG
GACCGTCGTGGAGAAGCGGTACTTGAAGATCACGGCCAATACTGATGGATTGCTGCTTCG
GTCGAAATTCGGTGAATGCATAGGTAAACATTGTAAATTTGAGGAATTGTTAACTAAATA
AATGGTTTGGATCTAACTTGGAATATTGATGACATATTGTAGGGATGAACTCAAAAGAAT
TTGCCTTGGAATTGTTTGACGCCTTAGCTAGAAAAAGTCACTTGAAAGGGGATGTGATTA
CTGAGACCGAGTTGAAGAAATTTTGGGAACAAATAAATGATAAAAGCTTTGATTCAAGGC
TTATCACGTTCTTTGACTTGTAATTTTTTCTAATCATCACACATTTTGGTCATTCGTTTC
ATTTCTTTGGGTCTTTACAAGTTTTGTAAAATGAATGTAAAGTTTGAGCCAAAGGGTTTG
TTTCAAATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CATCAATGAGTATGATTGTCGGTATGGGGAAAAAAAAAAAATAATTACC
   
Sequence Name 07-K106738-0022-306-B12C-8409
GenBank Accession LN489288 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106738-0022-306-B12C-8409
TTGAATAATCCTGCATTTGAAACAAACCCTTTGGCTCCACTTTACATTCATTTTACAAAA
CTTGTAAAGACCCAAAGAACTGAAACGAATGACCAAAATGTGTGATGATTAGAAAAAATT
ACAAGTCAAAGAACGTGATAAGCCTTGAATCAAAGCTTTTATCATTTATTTGTTCCCAAA
ATTTCTTCAACTCGGTCTCAGTAATCACATCCCCTTTCAAGTGACTTTTTCTAGCTAAGG
CGTCAAACAATTCCAAGGCAAATTCTTTTGAGTTCATCCCTACAATATGTCATCAATATT
CCAAGTTAGATCCAAACCATTTATTTAGTTAACAATTCCTCAAATTTACAATGTTTACCT
ATGCATTCACCGAATTTCGACCGAAGCAGCAATCCATCAGTATTGGCCGTGATCTTCAAG
TACCGCTTCTCCACGACGGTCCAGGCAGCGTTACCGTCGGTCTTACTGATGATCTTGAGC
CCTTTCAAGGCCTGACCGGCCGTTGACTTCGACCGGTCCAATCGAGGTGCCTTACTCTCT
CCAGATACCA

Last Updated on 06.12.2018 16:11