Sequences from confirmation amplicon


Confirmation sequences for line 306G03 and hit At1g31080 are shown.
Line ID 306G03
Locus At1g31080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11092610
Primers used for PCR BV14 - 8474, show primer details
   
Sequence Name 02-K105708-0022-306-G03C-BV14
GenBank Accession LN487034 [GenBank]
Sequencing primer gene specific primer, BV14: 5'-AAGTTCGGTAGAGTTGAGGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K105708-0022-306-G03C-BV14
GACTGAAATGTGAGGTGGAGGGAGATTATATGTCCCTTTTCATATGATCCTCCTCGGGAA
AAGCCGATATGCATCAATGGGGTTTTGTATTACATAAGTGTCGGTAGTTATTATTCACTT
ATAGGTTGCTTTGATGTTAGGTCTGAGAAATTCAAGTTTTTATATCTAAGCCCAGATTGC
TTTCCAAACTGGTCTAGAAAATTGATAAACTATAAGGGTAAATTAGGTGTGACTATAATG
GAAGATTATCATGGTGGATTTCCCCTTAAGTTGCGTATGTGGGTTCTAGAGGATGTCGAG
AAAGAGGAATGGACGAGATATGCCTACACTTTGAGGCCTGAGAATAAAGTCGAGGACAAT
GACTATGTATATGTTGTTGGGGCAACTGCTTCAGGTGAAATTGTTTTGACGAAACAGAAT
GCATATAAACCGTTTTATGTTTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 07-K105709-0022-306-G03C-8409
GenBank Accession LN489270 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105709-0022-306-G03C-8409
ATTGAATATATCCTGTAAAACATAAAACGGTTTATATGCATTCTGTTTCGTCAAAACAAT
TTCACCTGAAGCAGTTGCCCCAACAACATATACATAGTCATTGTCCTCGACTTTATTCTC
AGGCCTCAAAGTGTAGGCATATCTCGTCCATTCCTCTTTCTCGACATCCTCTAGAACCCA
CATACGCAACTTAAGGGGAAATCCACCATGATAATCTTCCATTATAGTCACACCTAATTT
ACCCTTATAGTTTATCAATTTTCTAGACCAGTTTGGAAAGCAATCTGGGCTTAGATATAA
AAACTTGAATTTCTCAGACCTAACATCAAAGCAACCTATAAGTGAATAATAACTACCGAC
ACTTATGTAATACAAAACCCCATTGATGCATATCGGCTTTTCCCGAGGAGGATCATATGA
AAAGGGACATATAATCTCCCTCCACCTCACATTTTCAGTTCCTAATGTCAGAATATGATA
AACCCATTCATCCTCAATCTNCCCGAAA

Last Updated on 06.12.2018 16:11