Sequences from confirmation amplicon


Confirmation sequences for line 307A12 and hit At5g05620 are shown.
Line ID 307A12
Locus At5g05620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1679939
Primers used for PCR 17vf - 8474, show primer details
   
Sequence Name 62-K117914-0022-307-A12C-17vf
GenBank Accession LN510847 [GenBank]
Sequencing primer gene specific primer, 17vf: 5'-AGCGGATGACCAACACTATATACC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117914-0022-307-A12C-17vf
TGGTATTCGAATGGTGAATACCGGAATCTCTACAATCATGAAAATATCTTCCTTTCTGAC
CATGGCGGTGGTGCTGGTAATAACTGGGCCAGTGGATATCACCAAGGGAAAGGTGTTGAA
GAGGAAATCATGGACATGATTGATCGTGAAGCTGATGGAAGTGACAGTCTGGAGGGTTTT
GTTCTTTGCCACTCTATTGCTGGAGGCACTGGCTCAGGTATTTTACTTTTTATTGCCAAT
CCCTATCTTCGTTTAGCTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCACCAATATTAT
   
Sequence Name 22-K117913-0022-307-A12C-8409
GenBank Accession LN496295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117913-0022-307-A12C-8409
GCTAAACGAAGATAGGGATTGGCAATAAAAAGTAAAATACCTGAGCCAGTGCCTCCAGCA
ATAGAGTGGCAAAGAACAAAACCCTCCAGACTGTCACTTCCGTCAGCTTCACGATCAATC
ATGTCCATGATTTCCTCTTCAACACCTTTCCCTTGGTGATATCCACTGGCCCATTTATTA
CCAGCACCACCGCCGTGGTAAGAAAGGAAGATATTTTCATGATTGTAGAGATTCCGGTAT
TCACCATTCTGAATACCATTGATAACTCTGGGCTCCAAATCAATGAGGAGAGCTCTTGGT
ATATAGTGTTGGTCATCCACTAGAAAAAAAAAGAGTCATTGCCCCTTTTTTTTC

Last Updated on 06.12.2018 16:11