Sequences from confirmation amplicon


Confirmation sequences for line 307C07 and hit At3g10660 are shown.
Line ID 307C07
Locus At3g10660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3332538
Primers used for PCR DL85 - 8474, show primer details
   
Sequence Name 12-K018754-022-307-C07C-DL85
GenBank Accession LN491365 [GenBank]
Sequencing primer gene specific primer, DL85: 5'-TCCTTCATGGAGATCTTGACTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K018754-022-307-C07C-DL85
CTGATCCATGGCCAAGCATCTCTGAAAGCGCGAAAGACTTGGTAAGGAAAATGCTTGTTC
GAGACCCCAAGCGAAGACTAACCGCACATCAAGTATTATGTAAGTTATTTAAGTCTAAAT
CATTCATCTTAGTTCTATTGAGCACAGAACATAACCTGGTATATCTTTTAACAATATGCT
AGTTGATTTTCACCGTTGTGTTTCTTCTCAAGGCGTTTTGTTTATGAACAGGTCATCCAT
GGGTACAGATTGACGGTGTGGCTCCAGATAAACCTCTGGACTCTGCTGTTCTGAGCCGTA
TGAAGCAATTTTCTGCAATGAACAAGTTCAAGAAAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGANAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 16-K018754-022-307-C07C-8409
GenBank Accession LN493232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018754-022-307-C07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTTTCTTGAACTTGTTCATTGCAGAAAATT
GCTTCATACGGCTCAGAACAGCAGAGTCCAGAGGTTTATCTGGAGCCACACCGTCAATCT
GTACCCATGGATGACCTGTTCATAAACAAAACGCCTTGAGAAGAAACACAACGGTGAAAA
TCAACTAGCATATTGTTAAAAGATATACCAGGTTATGTTCTGTGCTCAATAGAACTAAGA
TGAATGATTTAGACTTAAATAACTTACATAATACTTGATGTGCGGTTAGTCTTCGCTTGG
GGTCTCGAACAAGCATTTTCCTTACCAAGTCTTTCGCGCTTTCAGAGATGCTTGGCCATG
GATCAGATGAAAAGTCAAGATCTCCATGAAGG

Last Updated on 06.12.2018 16:11