Sequences from confirmation amplicon


Confirmation sequences for line 307C10 and hit At1g04810 are shown.
Line ID 307C10
Locus At1g04810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1350360
Primers used for PCR 16et - 8474, show primer details
   
Sequence Name 55-K110926-0022-307-C10C-16et
GenBank Accession LN508844 [GenBank]
Sequencing primer gene specific primer, 16et: 5'-TGATGCTGATCAAACTCTTCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K110926-0022-307-C10C-16et
AAATCAAAAAGGTCTCAGAAAGCACAAAAACAGTTTAAGATCTCAAGCAAAAGTCGAGTC
TCTACAACTTTCAAAAGAACAAATTTTCCGCATCATGACGGTAAGGGAGCGTCCTTAGCC
CAGATAACAATGGTTCTATTCAGAATCAACAGAATTAAGTAAAGCAATTCAATAAAGTTT
GTCACTTGTGAATTAACGCATTCAAATCTAACTCGACACAAGAGTGGAAAAACTTGGAAT
GTAATACCCTAACTAAGAAATATCAAGAGTGCTAAAACTCATCCAAATCCCATAAGCTTT
ACTCCCAGTAAGCAAACAGAATAGCCTCACGAAGGGAAACAATTAAAAAAAAGAGAAGAG
AGAATTGAATACATGATTGGGACACTAGTGGAGATCTCGGGCCAAAATTGGTCAACCAAT
CTGATGAGATATGAGAGAGCGTGAAGTTTCAAGGAAGGATGGCTTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 39-K110925-0022-307-C10C-8409
GenBank Accession LN503388 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K110925-0022-307-C10C-8409
TTGAAGCCATCCTTCCTTGAAACTTCACGCTCTCTCATATCTCTTCAGATTGGTTGACCA
ATTTTGGCCCGAGATCTCCACTAGTGTCCCAATCATGTATTCAATTCTCTCTTCTCTTTT
TTTTAATTGTTTCCCTTCGTGAGGCTATTCTGTTTGCTTACTGGGAGTAAAGCTTATGGG
ATTTGGATGAGTTTTAGCACTCTTGATATTTCTTAGTTAGGGTATTACATTCCAAGTTTT
TCCACTCTTGTGTCGAGTTAGATTTGAATGCGTTAATTCACAAGTGACAAACTTTATTGA
ATTGCTTTACTTAATTCTGTTGATTCTGAATAGAACCATTGTTATCTGGGCTAAGGACGC
TCCCTTACCGTCATGATGCGGAAAATTTGTTCTTTTGAAAGTTGTAGAGACTCTACTTTT
GCTTGAGATCTTAAACTGTTTTTGTGCTATTCTGAGACCTTTTTTTGATTTTTTTGTAGA
GAGAGCTTATACGAAGATGAAGAGTTTGATCCAGCATC

Last Updated on 06.12.2018 16:11