Sequences from confirmation amplicon


Confirmation sequences for line 307F10 and hit At1g33260 are shown.
Line ID 307F10
Locus At1g33260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12065086
Primers used for PCR 80cs - 8474, show primer details
   
Sequence Name 57-K108895-0022-307-F10C-80cs
GenBank Accession LN509443 [GenBank]
Sequencing primer gene specific primer, 80cs: 5'-TCGTAGTGGTTATAACGTCAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K108895-0022-307-F10C-80cs
CTTCCTCTTATCACAAACTGGTCAAACTCTGCCTCTCTCTTCTCTAAACCCAAACAAAAG
AAAAAAACCATTTTTCTCATCTTCTTCTTCTTTATTCACAAACCTCACCAAACACACATT
CCTTAAACCAGATCTTCATTCTCCGATTCTCTTGTCCCTTATTTGTCTGCTGTTACAAAA
ACCCTCTTCATTTTCCCAGAAACCCACTTATCTTCTTCTTAAATTACTATCATCCTCACT
CAAAAGTCTCACACTTTCTCAAAATGTCTTGTTTCTTAAGCTGTGTCCGTTTCGAAACAA
CCACCGACAACAACAAACCCACGAGTACAGAATCTGGTTCTGTCTCCGGTGTTACCTGTT
ATACCTGGGATGATGTTGAGTCTCTCACTTCCAATTTCTCTAGACTTATTGGTACTGGTG
GCTATAGTAGTATCTACTTAGCTCGTGTGTCTGGTTCTATCAACGCAGCTCTCAAGGTTC
ATGTCAGTAGTCATCGTCTTTATCAAGTGTTTAGGTCTGAGCTTGAGATTTTGCTTCGTC
TTCAACATCCTCACTTCAATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 41-K108894-0022-307-F10C-8409
GenBank Accession LN504105 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K108894-0022-307-F10C-8409
ATTTACATTGAATTATCCTGAATTGAAGTGAGGATGTTGAAGACGAAGCAAAATCTCAAG
CTCAGACCTAAACACTTGATAAAGACGATGACTACTGACATGAACCTTGAGAGCTGCGTT
GATAGAACCAGACACACGAGCTAAGTAGATACTACTATAGCCACCAGTACCAATAAGTCT
AGAGAAATTGGAAGTGAGAGACTCAACATCATCCCAGGTATAACAGGTAACACCGGAGAC
AGAACCAGATTCTGTACTCGTGGGTTTGTTGTTGTCGGTGGTTGTTTCGAAACGGACACA
GCTTAAGAAACAAGACATTTTGAGAAAGTGTGAGACTTTTGAGTGAGGATGATAGTAATT
TAAGAAGAAGATAAGTGGGTTTCTGGGAAAATGAAGAGGGTTTTTGTAACAGCAGACAAA
TAAGGGACAAGAGAATCGGAGAATGAAGATCTGGTTTAAGGAATGTGTGTTTGGTGAGGT
TTGTGAATAAAGAAGAAGAAGATGAGAAAAATGGTTTTTTTCTTTTGTTTGGGTTTAGAG
AAGAGAGAGGCAGAGTTTGACCAGTTTGTGATTAAGAGGAAGATGAAGAAGAAGAAGAAA
CCTTCCTTAGAAGCTGCACTGACGTTATAACCACTACGA

Last Updated on 06.12.2018 16:11